Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
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| Creator |
Khatik, Renuka
Dwivedi, Pankaj Reddy, V B Sharma, Komal Chuttani, Krishna Mishra, A K Dwivedi, A K |
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| Date |
2015-03-20T11:43:18Z
2015-03-20T11:43:18Z 2015 |
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| Identifier |
RSC Advances, 2015, 5(21), 16507-16520
http://hdl.handle.net/123456789/1435 |
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| Description |
Aim: The aim of the study was to develop a drug delivery system for specific targeting in colon cancer. We have developed Eudragit S-100 (ES) coated folic acid (FA) conjugated gliadin (Gd) delivery system for the effective targeting to the over expressed folate receptors (FRs) in colon cancer.
Method: The FA conjugate with Gd (FA-Gd) was synthesized and characterized by FTIR and 1H NMR, this developed conjugated was used to prepare curcumin (CU) loaded nanoparticles (NP) by desolvation method.
Result: FA-CU-GdNP was further coated with ES and ES-FA-CU-GdNP was obtained. The ES-FA-CU-GdNP were also capable of inducing cell caspase dependent apoptosis in Caco-2 cell lines and exhibit DNA intercalating activity. In therapeutic experiments the ES-FA-CU-GdNP was administered orally to HCT-116 tumor bearing nude mice. In vivo data of bio-distribution showed that ES-FA-CU-GdNP delivered maximum amount of NP in the colon and tumor, respectively after 12 hours, reflecting its targeting potential to the colon and tumor. Gamma scintigraphy study suggested that ES-FA-CU-GdNP at low pH and released NP slowly at pH 7.4 in the colon.
Conclusion: These studies provide evidences that ES-FA-CU-GdNP holds promise to address over-expressed FRs in colorectal cancer and were found to be safe for oral administration for prolonged duration.
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1189998 bytes
application/pdf |
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| Language |
en
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| Relation |
CSIR-CDRI Communication No. 8892
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| Subject |
Nanoparticles
Curcumin Nude mice apoptosis and colon cancer |
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| Title |
Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles
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| Type |
Article
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