HIV-1 Nef Breaches Placental Barrier in Rat Model
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Singh, Poonam
Agnihotri, S K Tewari, M C Kumar, Sadan Sachdev, Monika Tripathi, R K |
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| Date |
2013-06-18T08:40:22Z
2013-06-18T08:40:22Z 2012 |
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| Identifier |
http://hdl.handle.net/123456789/1066
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| Description |
The vertical transmission of HIV-1 from the mother to fetus is known, but the molecular mechanism regulating this transmission is not fully characterized. The fetus is highly protected by the placenta, which does not permit microbial
pathogens to cross the placental barrier. In the present study, a rat model was established to observe the effect of HIV-1
protein Nef on placental barrier. Evans blue dye was used to assay permeability of placental barrier and fourteen day
pregnant Sprague Dawley rats were injected intravenously with 2% Evans blue dye along with various concentrations of recombinant Nef. After an hour, animals were sacrificed and dye migration was observed through the assimilation of
peripheral blood into fetus. Interestingly, traces of recombinant Nef protein were detected in the embryo as well as amniotic fluid and amniotic membrane along with placenta and uterus. Our study indicates that recombinant HIV-1-Nef
protein breaches the placental barrier and allows the migration of Evans blue dye to the growing fetus. Further the concentration of Nef protein in blood is directly proportional to the intensity of dye migration and to the amount of Nef
protein detected in uterus, placenta, amniotic membrane, amniotic fluid and embryo. Based on this study, it can be concluded that the HIV-1 Nef protein has a direct effect on breaching of the placental barrier in the model we have
established in this study. Our observations will be helpful to understand the molecular mechanisms related to this breach of
placental barrier by Nef in humans and may be helpful to identify specific Nef inhibitors.
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| Format |
2806012 bytes
application/pdf |
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| Language |
en
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| Relation |
88-12-PLoS ONE 7(12): e51518
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| Subject |
HIV
Rat Placental |
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| Title |
HIV-1 Nef Breaches Placental Barrier in Rat Model
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| Type |
Article
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