Immunoadjuvant chemotherapy of visceral leishmaniasis in hamsters using Amphotericin B encapsulated nano-emulsion template based chitosan nanocapsules
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
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| Creator |
Asthana, Shalini
Jaiswal, A K Gupta, P K Pawar, V K Dube, Anuradha Chourasia, M K |
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| Date |
2013-07-16T09:48:56Z
2013-07-16T09:48:56Z 2013 |
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| Identifier |
Antimicrobial. Agents Chemotherapy. 2013, 57 (4), 1714-1722
http://hdl.handle.net/123456789/1087 |
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| Description |
The accessible treatment options for life-threatening neglected visceral leishmaniasis (VL) disease have problems regarding efficacy, stability, adverse effects and cost, making treatment a complex issue. Herein, we formulated nanometric amphotericin B (AmB) encapsulated chitosan-nanocapsules (CNC-AmB) using polymer deposition technique mediated by nano-emulsion template fabrication. CNC-AmB exhibited good steric stability in vitro where chitosan content was found to be efficient in preventing their destabilization in the presence of protein and Ca+2. Toxicity study on J774A model cell line and erythrocytes revealed less toxicity of CNC-AmB compared to commercialized AmB formulations such as Fungizone® and AmBisome®. Experimental results of in vitro (macrophage amastigote system, IC50 = 0.19±0.04 µg AmB/ml) and in vivo (Leishmania donovani infected hamsters, 86.1±2.08% parasite inhibition) in conjunction with effective internalization by macrophages illustrated the efficacy of CNC-AmB to augment antileishmanial property. Quantitative mRNA analysis by RT-PCR showed that improved effect was synergized with up regulated Tumor Necrosis Factor-α (TNF-α), Interleukin-12 (IL-12) and inducible nitric oxide synthase, and down regulated Transforming Growth Factor-β (TGF- β), IL-10 and IL-4. These research findings suggest that developed cost-effective CNC-AmB immunoadjuvant chemotherapeutic delivery system could be a viable alternative to the current high-cost commercial lipid based formulations
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2076572 bytes
application/pdf |
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| Language |
en
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| Relation |
CSIR-CDRI Communication No. 8384
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| Subject |
Nanocapsules
Nano-emulsion Chitosan Macrophages Cytokines |
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| Title |
Immunoadjuvant chemotherapy of visceral leishmaniasis in hamsters using Amphotericin B encapsulated nano-emulsion template based chitosan nanocapsules
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| Type |
Article
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