Molecular Cloning and Characterization of Glucose-6-phosphate dehydrogenase from Brugia malayi
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
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| Creator |
Verma, Anita
Suthar, M K Doharey, P K Gupta, Smita Yadav, Sunita Chauhan, P M S Saxena, J K |
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| Date |
2013-10-08T11:50:35Z
2013-10-08T11:50:35Z 2013 |
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| Identifier |
Parasitology, 2013, 140 (7), 897-906
http://hdl.handle.net/123456789/1134 |
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| Description |
Glucose 6-phosphate dehydrogenase (G6PD) a regulatory enzyme of pentose phosphate pathway from Brugia malayi was cloned, expressed and biochemically characterised. The Km values for glucose 6-phosphate and NADP were 0.25 mM and 0.014mM respectively. The rBmG6PD exhibited optimum pH of 8.5 and temperature, 40°C. ATP--S, ATP-β,-NH, ADP-β-S, Na+, K+, Li+ and, Cu++ ions were found to be strong inhibitors of rBmG6PD. The rBmG6PD, a tetramer with subunit molecular weight of 75 kDa contains 0.02 mol of SH group per mol of monomer. Blocking of SH group with SH-inhibitors, leads to activation of rBmG6PD activity by N-ethylmaleimide. CD analysis indicated that rBmG6PD is composed of 37% α-helices and 26% β-sheets. The unfolding equilibrium of rBmG6PD with GdmCl/urea showed the triphasic unfolding pattern along with the highly stable intermediate obtained by GdmCl.
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5397663 bytes
application/pdf |
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| Language |
en
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| Relation |
CSIR- CDRI Communication No. 8392
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| Subject |
Brugia malayi
Glucose 6-phosphate dehydrogenase DHEA Inhibitors Circular Dichroism GdmCl Urea Drug target |
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| Title |
Molecular Cloning and Characterization of Glucose-6-phosphate dehydrogenase from Brugia malayi
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| Type |
Article
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