A novel chromo- and fluorogenic dual sensor for Mg2+ and Zn2+ with cell imaging possibilities and DFT studies
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
A novel chromo- and fluorogenic dual sensor for Mg2+ and Zn2+ with cell imaging possibilities and DFT studies
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| Creator |
Alam, Rabiul
Mistri, Tarun Katarkar, Atul Chaudhuri, Keya Mandal, Sushil Kumar Khuda-Bukhsh, Anisur Rahman Das, Kalyan K Ali, Mahammad |
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| Subject |
Molecular & Human Genetics
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| Description |
A diformyl-p-cresol (DFC)-8-aminoquinoline based dual signaling probe was found to exhibit colorimetric
and fluorogenic properties on selective binding towards Mg2+ and Zn2+. Turn-on fluorescent enhancements (FE) as high as 40 fold and 53 fold in 9 : 1 MeCN/water (v/v) at pH 7.2 in HEPES buffer for Mg2+ and Zn2+, respectively, were observed. The binding constants determined from the fluorescence titration data are: K ¼ (1.52 � 0.21) � 105 M�1 and (9.34 � 4.0) � 103 M�2 at n ¼ 1 and 0.5, for Mg2+ and
Zn2+, respectively. The L : M binding ratios were also determined by Job's method, which support the
above findings. This is further substantiated by HRMS analysis. Due to solubility in mixed organo-aqueous
solvents as well as cell permeability it could be used for the in vitro/in vivo cell imaging of Mg2+ and Zn2+
ions with no or negligible cytotoxicity. This probe could be made selective towards Mg2+ over Zn2+ in the presence of TPEN, both under intra- and extracellular conditions and is superior to other Mg2+ probes which suffer from selectivity of Mg2+ over Ca2+ or Zn2+. Furthermore the dissociation constant (Kd ¼ 6.60 mM) of the Mg2+-(DFC-8-AQ) complex is far lower than the so far reported Mg2+ probes which fall in the mM range
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2133/1/ANALYST__V_139_(_16)4022%2D4030;2014[59].pdf
Alam, Rabiul and Mistri, Tarun and Katarkar, Atul and Chaudhuri, Keya and Mandal, Sushil Kumar and Khuda-Bukhsh, Anisur Rahman and Das, Kalyan K and Ali, Mahammad (2014) A novel chromo- and fluorogenic dual sensor for Mg2+ and Zn2+ with cell imaging possibilities and DFT studies. Analyst, 139 (16). pp. 4022-4030. |
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| Relation |
htp://dx.doi.org.10.1039/c3an02347h
http://www.eprints.iicb.res.in/2133/ |
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