Vesicular transport of a ribonucleoprotein to mitochondria
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Vesicular transport of a ribonucleoprotein to mitochondria
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| Creator |
Mukherjee, Joyita
Mahato, Biraj Adhya, Samit |
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| Subject |
Molecular & Human Genetics
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| Description |
Intracellular trafficking of viruses and proteins commonly occurs via the early endosome in a process involving Rab5. The RNA Import Complex (RIC)-RNA complex is taken up by mammalian cells and targeted to mitochondria. Through RNA interference, it was shown that mito-targeting of the ribonucleoprotein (RNP) was dependent on
caveolin 1 (Cav1), dynamin 2, Filamin A and NSF. Although a minor fraction of the RNP was transported to endosomes in a Rab5- dependent manner, mito-targeting was independent of Rab5 or other endosomal proteins, suggesting that endosomal uptake and mitotargeting occur independently. Sequential immunoprecipitation of the cytosolic vesicles showed the sorting of the RNP away fromCav1 in a process that was independent of the endosomal effector EEA1 but sensitive to nocodazole. However, the RNP was in two types of
vesicle with or without Cav1, with membrane-bound, asymmetrically orientated RIC and entrapped RNA, but no endosomal components, suggesting vesicular sorting rather than escape of free RNP fromendosomes. In vitro, RNP was directly transferred from the Type 2 vesicles to mitochondria. Live-cell imaging captured spherical Cav12
RNP vesicles emerging from the fission of large Cav+ particles. Thus, RNP appears to traffic by a different route than the classical Rab5- dependent pathway of viral transport
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2137/1/BIOLOGY_OPEN___3(11)__1083%2D1091_;2014.pdf
Mukherjee, Joyita and Mahato, Biraj and Adhya, Samit (2014) Vesicular transport of a ribonucleoprotein to mitochondria. Biology Open, 3 (11). pp. 1083-1091. |
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| Relation |
http://dx.doi.org/10.1242/bio.20149076
http://www.eprints.iicb.res.in/2137/ |
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