Targeting Proteins with Toxic Azo Dyes: A Microcalorimetric Characterization of the Interaction of the Food Colorant Amaranth with Serum Proteins
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Targeting Proteins with Toxic Azo Dyes: A Microcalorimetric
Characterization of the Interaction of the Food Colorant Amaranth with Serum Proteins
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| Creator |
Basu, Anirban
Kumar, Gopinatha Suresh |
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| Subject |
Chemistry
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| Description |
The interaction of amaranth with two homologous serum albumins from human and bovine (HSA and BSA) was
studied by microcalorimetry. The binding stoichiometry for the complexation of amaranth to both BSA and HSA was around 1, and the equilibrium constants were (5.79 ± 0.07) × 105 and (1.76 ± 0.05) × 105 M−1, respectively. The binding reaction to HSA at 298.15 K was driven by a large negative enthalpic contribution and a small but positive entropic contribution, while to BSA, it was entirely enthalpy-driven and the entropic contribution was unfavorable. Parsing of the standard molar Gibbs energy revealed that the complexation was dominated by non-polyelectrolytic forces. Temperature-dependent isothermal titration calorimetry
studies revealed that the enthalpic contribution increased and the entropic contribution decreased with the rise in the
temperature but the Gibbs energy change remained almost unaltered. Differential scanning calorimetry results revealed that the binding reaction stabilized the serum albumins significantly against thermal unfolding
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| Publisher |
American Chemical Society
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2151/1/JOURNAL_OF_AGRICULTURAL_AND_FOOD_CHEMISTRY__V_62(_31)_7955%2D7962;2014[63].pdf
Basu, Anirban and Kumar, Gopinatha Suresh (2014) Targeting Proteins with Toxic Azo Dyes: A Microcalorimetric Characterization of the Interaction of the Food Colorant Amaranth with Serum Proteins. Journal of Agricultural and Food Chemistry, 62 (31). pp. 7955-7962. |
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| Relation |
http://dx.doi.org/10.1021/jf5025278
http://www.eprints.iicb.res.in/2151/ |
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