Comparative evaluation of genotoxicity by micronucleus assay in the buccal mucosa over comet assay in peripheral blood in oral precancer and cancer patients
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Comparative evaluation of genotoxicity by micronucleus assay in the buccal mucosa over comet assay in peripheral blood in oral precancer and cancer patients
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| Creator |
Katarkar, Atul
Mukherjee, Sanjit Kha, Masood H Ray, Jay Gopal Chaudhuri, Keya |
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| Subject |
Molecular & Human Genetics
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| Description |
Early detection and quantification of DNA damage in oral
premalignancy or malignancy may help in management of
the disease and improve survival rates. The comet assay
has been successfully utilised to detect DNA damage in oral
premalignant or malignancy. However, due to the invasive
nature of collecting blood, it may be painful for many
unwilling patients. This study compares the micronucleus
(MN) assay in oral buccal mucosa cells with the comet
assay in peripheral blood cells in a subset of oral habitinduced precancer and cancer patients. For this, MN assay of exfoliated epithelial cells was compared with comet assay of peripheral blood leucocytes among 260 participants, including those with oral lichen planus (OLP; n = 52), leukoplakia (LPK; n = 51), oral submucous fibrosis (OSF;n = 51), oral squamous cell carcinoma (OSCC; n = 54) and normal volunteers (n = 52). Among the precancer groups,
LPK patients showed significantly higher levels of DNA
damage as reflected by both comet tail length (P < 0.0001)
and micronuclei (MNi) frequency (P = 0.0009). The DNA
damage pattern in precancer and cancer patients was OLP
< OSF < LPK < OSCC, and with respective oral habits, it
was multiple habits > cigarette + khaini > cigarette smokers
> areca + khaini > areca. There was no significant difference in the comet length and MNi frequency between males and females who had oral chewing habits. An overall significant correlation was observed between MNi frequency
and comet tail length with r = 0.844 and P < 0.0001. Thus,
the extent of DNA damage evaluation by the comet assay in
peripheral blood cells is perfectly reflected by the MN assay on oral exfoliated epithelial cells, and MNi frequency can be used with the same effectiveness and greater efficiency in early detection of oral premalignant conditions.
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| Publisher |
Oxford University Press
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2160/1/MUTAGENESIS__V_29(_5_)_325%2D334;2004[47].pdf
Katarkar, Atul and Mukherjee, Sanjit and Kha, Masood H and Ray, Jay Gopal and Chaudhuri, Keya (2014) Comparative evaluation of genotoxicity by micronucleus assay in the buccal mucosa over comet assay in peripheral blood in oral precancer and cancer patients. Mutagenesis, 29 (5). pp. 325-334. |
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| Relation |
http://dx.doi.org/10.1093/mutage/geu023
http://www.eprints.iicb.res.in/2160/ |
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