Efficacy of Withania somnifera chemotypes NMITLI – 101R, 118R and Withaferin A against experimental visceral leishmaniasis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Efficacy of Withania somnifera chemotypes NMITLI – 101R, 118R and Withaferin A against experimental visceral leishmaniasis
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| Creator |
TRIPATHI, C. D. P
GUPTA, R KUSHAWAHA, P. K MANDAL, C MISRA BHATTACHARYA, S Dube, A |
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| Subject |
Cancer Biology and Inflammatory Disorder Division
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| Description |
The immunoprophylactic and therapeutic potentials of root
extracts of Withania somnifera chemotypes (NMITLI-118,
NMITLI-101) and pure withanolide–withaferin A was investigated against Leishmania donovani infection in hamsters.The naive animals, fed orally with immunostimulatory doses of chemotypes 101R, 118R (10 and 3 mg/kg) and withaferin A (9 and 3 mg/kg) for five consecutive days and challenged with Leishmania parasites on day 6, were euthanized on days 30 and 45 p.c. for the assessment of parasite clearance, real-time analysis of mRNAs of Th1/Th2 cytokines (IFN-c, IL-12, TNF-a, iNOS/IL-4, IL-10 and TGF-b), NO production, reactive oxygen species(ROS) generation, lymphocyte transformation test and antibody responses. By day 45 p.c., there was a significant increase in the mRNA expression of iNOS, IFN-c, IL-12 and TNF-a but decrease in IL-4, IL-10 and TGF-b, an enhanced Leishmania-specific LTT response as well as ROS, NO and antileishmanial IgG2 levels in 101R-treated hamsters followed by 118R- and withaferin A-treated ones, respectively. When these chemotypes were given to L. donovani-infected hamsters at different doses, there was moderate therapeutic efficacy of chemotype 101R (~50%) at 30 mg/kg 9 5 followed by the other two. The results established that the 101R is the most potential chemotype and can be evaluated for combination therapy along with available antileishmanials.
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| Publisher |
Blackwell Publishing
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2181/1/PARASITE_IMMUNOLOGY__V_36__(_6)_253%2D265_;2014[94].pdf
TRIPATHI, C. D. P and GUPTA, R and KUSHAWAHA, P. K and MANDAL, C and MISRA BHATTACHARYA, S and Dube, A (2014) Efficacy of Withania somnifera chemotypes NMITLI – 101R, 118R and Withaferin A against experimental visceral leishmaniasis. Parasite Immunology, 36 (6). pp. 253-265. |
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| Relation |
http://dx.doi.org/10.1111/pim.12112
http://www.eprints.iicb.res.in/2181/ |
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