Ellagic Acid, a Dietary Polyphenol, Inhibits Tautomerase Activity of Human Macrophage Migration Inhibitory Factor and Its Pro-inflammatory Responses in Human Peripheral Blood Mononuclear Cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Ellagic Acid, a Dietary Polyphenol, Inhibits Tautomerase Activity of Human Macrophage Migration Inhibitory Factor and Its Pro-inflammatory Responses in Human Peripheral Blood Mononuclear Cells
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| Creator |
Sarkar, Souvik
Siddiqui, Asim A Mazumder, Somnath De, Rudranil Saha, Shubhra J. Banerjee, Chinmoy Iqbal, Mohd. S Adhikari, Susanta Alam, Athar Roy, Siddhartha Bandyopadhyay, Uday |
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| Subject |
Infectious Diseases and Immunology
Structural Biology & Bioinformatics |
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| Description |
Ellagic acid (EA), a phenolic lactone, inhibited tautomerase activity of human macrophage migration inhibitory factor (MIF) noncompetitively (Ki = 1.97 ± 0.7 μM). The binding of EA to MIF was determined by following the quenching of tryptophan fluorescence. We synthesized several EA derivatives, and their structure−activity relationship studies indicated that the planar conjugated lactone moiety of EA was essential for MIF inhibition. MIF induces nuclear translocation of NF-κB and chemotaxis of peripheral blood mononuclear cells (PBMCs) to promote inflammation. We were interested in evaluating the
effect of EA on nuclear translocation of NF-κB and chemotactic activity in human PBMCs in the presence of MIF. The results showed that EA inhibited MIF-induced NF-κB nuclear translocation in PBMCs, as evident from confocal immunofluorescence microscopic data. EA also inhibited MIF-mediated chemotaxis of PBMCs. Thus, we report MIF-inhibitory activity of EA and inhibition of MIF-mediated proinflammatory responses in PBMCs by EA.
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| Publisher |
American Chemical Society
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2220/1/JOURNAL_OF_AGRICULTURAL_AND_FOOD_CHEMISTRY__V._63__(_20_)_4988%2D4998_;2015[13].pdf
Sarkar, Souvik and Siddiqui, Asim A and Mazumder, Somnath and De, Rudranil and Saha, Shubhra J. and Banerjee, Chinmoy and Iqbal, Mohd. S and Adhikari, Susanta and Alam, Athar and Roy, Siddhartha and Bandyopadhyay, Uday (2015) Ellagic Acid, a Dietary Polyphenol, Inhibits Tautomerase Activity of Human Macrophage Migration Inhibitory Factor and Its Pro-inflammatory Responses in Human Peripheral Blood Mononuclear Cells. Journal of Agricultural and Food Chemistry, 63 (20). pp. 4988-4998. |
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| Relation |
http://dx.doi.org/10.1021/acs.jafc.5b00921
http://www.eprints.iicb.res.in/2220/ |
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