A potent tumoricidal co-drug ‘Bet-CA’ - an ester derivative of betulinic acid and dichloroacetate selectively and synergistically kills cancer cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
A potent tumoricidal co-drug ‘Bet-CA’ - an ester derivative of betulinic acid and dichloroacetate selectively and synergistically kills cancer cells
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| Creator |
Saha, Suchandrima
Ghosh, Monisankar Dutta, Samir Kumar |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Selective targeting of cancer cells employing multiple combinations as co-drug holds promise for new generation therapeutics. Betulinic acid (BA), a plant secondary metabolite kills cancer cells and Dichloroacetate (DCA) is capable of reversing the Warburg phenotype by inhibiting pyruvate dehydrogenase kinase (PDK). Here, we report synthesis, characterization and tumoricidal potential of a co-drug Bet-CA, where a DCA molecule has been appended on C-3 hydroxyl group of BA to generate an ester derivative for increased solubility and subsequent cleavage by internal esterase(s) to release one unit each of BA and DCA. In vitro studies revealed pronounced synergistic cytotoxicity of Bet-CA against a broad spectrum of cancer cells and it selectively killed them when co-cultured with human fibroblasts. Bet-CA treatment increased reactive oxygen species (ROS) production, significantly altered mitochondrial membrane potential gradient (ΔΨm); followed by the release of cytochrome c (Cyt c) which prompted cells to undergo mitochondria mediated apoptosis. In vivo experimentation expectedly exhibited tumor inhibitory potential of Bet-CA and clinically achievable doses did not produce any apparent toxicity. Taken together, results suggestively raise an important corollary hypothesis stating that Bet-CA selectively and synergistically combats cancer without producing toxic manifestations and emerges to be the prospect for the new generation therapeutics
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/msword
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| Identifier |
http://www.eprints.iicb.res.in/2257/1/SCIENTIFIC_REPORTS__V._5_____Article_Number_7762;2015[80].docx
Saha, Suchandrima and Ghosh, Monisankar and Dutta, Samir Kumar (2015) A potent tumoricidal co-drug ‘Bet-CA’ - an ester derivative of betulinic acid and dichloroacetate selectively and synergistically kills cancer cells. Scientific Reports, 5 (7762). |
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| Relation |
htto://dx.doi.org/10.1038/srep07762
http://www.eprints.iicb.res.in/2257/ |
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