N-glycolylneuraminic acid specific lectin from Pila globosa snail
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
N-glycolylneuraminic acid specific lectin from Pila globosa snail
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| Creator |
Swarnakar, Snehasikta
Chowdhury, Partha Sarathi Sarkar, Manju |
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| Subject |
Cell Biology & Physiology
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| Description |
A N-glycolylneuraminic acid-specific lectin (PAL) has been
purified from an albumin gland extract of the apple snail, Pila globosa. Purification is conducted on a bovine submaxillary mucin-~$ar~s%-TB affinity matrix followed by gel filtration on a Sepharose 6B column. The lectin agglutinatz; rabbit erythrocytes. The hemagglutination activity is dependent on Ca concentration in a significant manner but with a remark able behaviour. The lectin is a trimeric glycoprotein of native Mr 440 kDa with 25% carbohydrate and is composed of three nonidentical subunits
of molecular weights 190, 145. and 105 kDa. It has a p1 of 7.0. The lectin exhibits a unique and strict specificity toward N-glycolylneuraminic
acid and this phenomenon discriminates it from other known sialic acid binding lectins. The uniqueness indicates the absolute need for a glycolyl substitution on the amino residue and of a glyceryl side chain on the exocyclic part and an axial-COOH group in neuraminic acid. The presence
of an acetyl
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| Publisher |
Elsevier
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| Date |
1991
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2282/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__V._178_(_1)85%2D94;1991.pdf
Swarnakar, Snehasikta and Chowdhury, Partha Sarathi and Sarkar, Manju (1991) N-glycolylneuraminic acid specific lectin from Pila globosa snail. Biochemical and Biophysical Research Communications, 178 (1). pp. 85-94. |
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| Relation |
http://www.eprints.iicb.res.in/2282/
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