Lipid changes Of goat sperm plasma membrane during epididymal maturation
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Lipid changes Of goat sperm plasma membrane during epididymal maturation
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| Creator |
Rana, Ajay P.S
Majumder, Gopal Chandra Misra, Suniti Ghosh, Amitabha |
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| Subject |
Cell Biology & Physiology
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| Description |
Highly purified plasma membranes of maturing goat caput-, corpus- and cauda-epididymal spermatozoa were isolated by
aqueous two-phase polymer methods and their lipid constituents were analysed, Phospholipid (app~'ox. 75% w/w),
neutral lipid (approx. 15% w/w) and glycolipid (approx. 10% w/w) were the major sperm membrane lipids. "l'here was
a significant decrease in the total lipids (approx. 25% w/w), phospholipid {approx. 30% w/w) and glycotipid (approx.
80% w/w) contents of sperm membrane during epididymal maturation. On the contrary, the mature cauda-sperrn
membrane showed greater (approx. 50% w/w) neutral lipid content than that of the immature caput sperm.
Phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin were the phospholipids of the sperm membrane, the former two being the major lipids. Both PC and PE fractions consisted of three species - diacyl, alkylacyl and alkenylacyl forms, the last one being lhe dominant species in both PC and PIE. Of all the phospholipids,
diacyl PE decreased most strikingly (approx. 65% w/w) during sperm maturation. The neutral lipid fraction contained sterols, wax esters,l-O alkyl-2,3-diacylglycerol, triacylglycerol and fail?, acids. Sterols represented nearly 75% w/w of the neutral lipids and cholesterol was the major component (approx. 95% w/w) of the sterol fraction. The sperm maturity was associated with marked increase of sterol {approx. 60% w/w) and steryl ester (approx. 200% w/w) and decrease (approx. 50-65% w/w) of the other membrane-bound neutral iipids, The glyeolipid was identified as monogalaetosyldiacylglycerol. The fatty acid profile of the various membrane lipids underwent marked alteration during the epididymal transit of the male gametes. Cholesterol/phospbolipid and saturated/unsaturated fatty acid ratios increased greatly in the maturing sperm membrane. The altered lipid profile of ',he ma~nre sperm membrane leads to changes in its fluidity that play an important role in determining the structure and functions of the biomembrane.
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| Publisher |
Elsevier
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| Date |
1991
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2284/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA__V._1061__(_2__)_185%2D196_;1991.pdf
Rana, Ajay P.S and Majumder, Gopal Chandra and Misra, Suniti and Ghosh, Amitabha (1991) Lipid changes Of goat sperm plasma membrane during epididymal maturation. BBA - Biochimica et Biophysica Acta, 1061 (2). pp. 185-196. |
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| Relation |
http://www.eprints.iicb.res.in/2284/
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