Goat sperm membrane: lectin-binding sites of sperm surface and lectin affinity chromatography of the mature sperm membrane antigens
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Goat sperm membrane: lectin-binding sites of sperm surface
and lectin affinity chromatography of the mature sperm
membrane antigens
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| Creator |
Sarkar, Madhumita
Majumder, Gopal Chandra Chatterjee, Tapati |
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| Subject |
Cell Biology & Physiology
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| Description |
The cell surface glycoproteins of goat epididymal maturing sperma~r;zoa have been investigated using lectins as
surface probes that interact with specific sugars with high a~,aity. Concanavalin A (ConA) and wheat-germ
agglutinin (WGA) showed high affinity for mature cauda epidi~ymal sperm ~gg[utination, whereas RCA,, kidney
beans lectin and peanut agglutinin caused much lower or little aggluth,~fion of the cells. The mature sperm
exhibited markedly higher efficacy than the immature caput epididymal sperm fo)r binding both ConA and WGA, as
evidenced by sperm agglutination and the binding of the fluorescence isothiocyanate (FITC)-Iabeiled lecfins.
FITC-ConA binds uniformly to the entire mature sperm surface whereas FITC.WGA binds to the acrosemai cap
region of the head. The FITC-RCA 2 mainly labelled the posterior head of mature cauda sperm. However. no
WGA-speciflc glycoprotein receptors could be detected in sperm plasma membrane (PM) by WGA-Sepharose
affinity chromatography. The data implied that the epididymal sperm maturation is associated with a marked
increase in the ConA/WGA receptors and that WGA receptors may be glycolipids rather than glyco~r~teins.
Analysis of the ConA receptors of cauda sperm PM identified by ConA-S:pbarose affinity chromatography and
subsequent resolution in SDS-PAGE demonstrated the presence of five glycopolypeptides of different concentrations
(98, 96, 43, 27 and 17 kDa) of goat sperm membrane. The immunobbt of these ConA-specific glycopeptides with
anti-sperm membrane antiserum showed that 98- and 96-kDa receptors are immunor~spo~sive.
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| Publisher |
Elsevier
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| Date |
1991
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2285/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA__V._1070___(_1_)_198%2D204;1991.pdf
Sarkar, Madhumita and Majumder, Gopal Chandra and Chatterjee, Tapati (1991) Goat sperm membrane: lectin-binding sites of sperm surface and lectin affinity chromatography of the mature sperm membrane antigens. BBA - Biochimica et Biophysica Acta, 1070 (1). pp. 198-204. |
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| Relation |
http://www.eprints.iicb.res.in/2285/
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