Purification and Characterization of Two Major Lectins from Araucaria brasiliensis syn. Araucaria angustifolia Seeds (Pinhao)1
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Purification and Characterization of Two Major Lectins from
Araucaria brasiliensis syn. Araucaria angustifolia Seeds (Pinhao)1
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| Creator |
Datta, Pradip K
Figueroa, Maria 0. D. C. R Lajolo, Franco M |
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| Subject |
Chemistry
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| Description |
Two major lectins (lectin I and lectin 11) were purified to homogeneity from the seeds of Araucaria brasiliensis (Gymnospermae).The purity of the lectins was confirmed by polyacrylamide gel electrophoresis, isoelectric focusing, and high performance liquid chromatography. They are glycoproteins in nature containing 6.3 and 2.9%, respectively, of neutral sugar and have absorption
coefficients of 3.8 and 4.7, respectively, at 280 nanometers.The molecular weights of both lectins obtained by gel filtration on Sephacryl S-400 were equal: 200,000. After dissociation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, molecular weights were 20,000 and 34,000, respectively, for lectin I and lectin 11, suggesting they are decameric and hexameric in nature. The amino acid composition of both lectins showed little
difference, but both had high amounts of acidic amino acids and lacked methionine in their molecule. The carbohydrate binding specificity of lectins was directed towards mannose, glucose, and their oligomers. High inhibitory activity was also found with thyroglobulin. The erythroagglutinating activity of the lectins was
enhanced in the presence of high-molecular-weight substances
both at 37 and 40C. Divalent cations do not appear to be essential for activity. They maintained their agglutinating activity over a broad but different range of pH: 5.5 to 7.5 and 6.5 to 7.5, respectively.Both lectins agglutinated erythrocytes of human ABO blood
types equally well.
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| Publisher |
American Society of Plant Biologists
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| Date |
1991
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2304/1/PLANT_PHYSIOLOGY__V._97_(_3__)_856%2D862;1991.pdf
Datta, Pradip K and Figueroa, Maria 0. D. C. R and Lajolo, Franco M (1991) Purification and Characterization of Two Major Lectins from Araucaria brasiliensis syn. Araucaria angustifolia Seeds (Pinhao)1. Plant Physiology, 97. pp. 856-862. ISSN 0032-0889 |
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| Relation |
http://www.eprints.iicb.res.in/2304/
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