Functions of interleukin-8 are mediated through thiol group(s) of IL-8 receptor in human polymorphonuclear neutrophils Effects of 5,5’-dithio-bis(2-nitrobenzoic acid) on IL-8 receptor
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Functions of interleukin-8 are mediated through thiol group(s) of IL-8 receptor in human polymorphonuclear neutrophils
Effects of 5,5’-dithio-bis(2-nitrobenzoic acid) on IL-8 receptor
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| Creator |
Dutta, Supriti
Ali, Esahak Samanta, Ajoy K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Interleukm-8, a neutrophil chemotacttc agent causes excesstve accumulation of the cells in a number of inflammatory diseases. The activity has been shown to be mediated through a specific functional receptor present on the surface of neutrophils. No information is available about the ammo acids constitutmg the IL-8 binding domain of the receptor. Treatment of neutrophtls with 5.5’-dtthio-bts(3-mtrobenzotc acid), a thiol-specific modifier. at the concentrattons of 0.4 mM and 1 mM reduced IL-8 binding ability and IL-g-induced migration of the cells by 45% and 65%.respectively. Dithiothrettol could regenerate the bmding capacity and the ligand could protect the receptor from the effect of the reagent. All the evidence suggests that one or more critical thiol residues are located m the IL-8 bmdmg site of the receptor which are mdtspensible for normal functions of IL-8.
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| Publisher |
Elsevier
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| Date |
1993
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
application/pdf |
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| Identifier |
http://www.eprints.iicb.res.in/2313/1/FEBS_LETTERS__V._325__(_3_)_262%2D266;1993[36].pdf
http://www.eprints.iicb.res.in/2313/2/FEBS_LETTERS__V._325__(_3_)_262%2D266;1993[36].pdf Dutta, Supriti and Ali, Esahak and Samanta, Ajoy K (1993) Functions of interleukin-8 are mediated through thiol group(s) of IL-8 receptor in human polymorphonuclear neutrophils Effects of 5,5’-dithio-bis(2-nitrobenzoic acid) on IL-8 receptor. FEBS Letters, 325 (3). pp. 262-266. |
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| Relation |
http://www.eprints.iicb.res.in/2313/
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