Forskolin Production in Untransformed Root Culture of Coleus ForskohlII
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Forskolin Production in Untransformed Root Culture of Coleus ForskohlII
|
|
| Creator |
Sen, Jayanti
Sharma, A. K Sahu, N P Mahato, Shashi B |
|
| Subject |
Chemistry
|
|
| Description |
Untransformed cultures of Coleus forskohlii Briq. were initiated from in vitro micropropagated shoots
derived from shoot tip culture. Effects of IAA, IBA and two IAA derivatives, 4-Cl-IAA and 5,6-Cl,-&4 were compared for growth and forskolin production in cultured roots grown on different basal media under different culture regimes. The roots grew well in the presence of all dosages of 4-Cl-IAA and 5,6-Cl,-IAA with 25% MS plus 1% sucrose and produced more forskolin as compared with IAA or IBA. The optimum concentrations needed for forskolin production were 1 pg l- ’ for 5,6-Cl,-IAA and 50 pg 1-l for 4-Cl-IAA. The highest amount of forskolin (0.09%) was raised at 50 pg l- ’ 4-Cl-IAA after 60 days of culture. This enhanced level of forskolin was almost equal to the content in the wild plant. However, under similar conditions and with 500 pg 1-l IAA or IBA, the amount of forskolin produced was much smaller. IAA derivative-induced production of forskolin indicates the possibility for scaling up of the production of this compound in untransformed root culture.
|
|
| Publisher |
Elsevier
|
|
| Date |
1993
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/2320/1/PHYTOCHEMISTRY__V._34__(_5_)_1309%2D1312;1993[12].pdf
Sen, Jayanti and Sharma, A. K and Sahu, N P and Mahato, Shashi B (1993) Forskolin Production in Untransformed Root Culture of Coleus ForskohlII. Phytochemistry, 34 (5). pp. 1309-1312. |
|
| Relation |
http://www.eprints.iicb.res.in/2320/
|
|