Transient overexpression ofWerner protein rescues starvation induced autophagy inWerner syndrome cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Transient overexpression ofWerner protein rescues starvation induced autophagy inWerner syndrome cells
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| Creator |
Maity, Jyotirindra
Bohr, Vilhelm A Laskar, Aparna Karmakar, Parimal |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Reduced autophagy may be associated with normal and pathological aging. Here we report a link between
autophagy and Werner protein (WRNp), mutated in Werner syndrome, the human premature aging Werner
syndrome (WS).WRNmutant fibroblast AG11395 and AG05229 respondweakly to starvation induced autophagy
compared to normal cells. While the fusion of phagosomes with lysosome is normal, WS cells contain fewer
autophagy vacuoles. Cellular starvation autophagy in WS cells is restored after transfection with full length
WRN. Further, siRNA mediated silencing of WRN in the normal fibroblast cell line WI-38 results in decreased
autophagy and altered expression of autophagy related proteins. Thus, our observations suggest that WRN may
have a role in controlling autophagy and hereby cellular maintenance.
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| Publisher |
Elsevier
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2401/1/Biochimica_et_Biophysica_Acta_1842_(2014)_2387%E2%80%932394.pdf
Maity, Jyotirindra and Bohr, Vilhelm A and Laskar, Aparna and Karmakar, Parimal (2014) Transient overexpression ofWerner protein rescues starvation induced autophagy inWerner syndrome cells. BBA - Biochimica et Biophysica Acta, 1842. pp. 2387-2394. |
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| Relation |
http://dx.doi.org/10.1016/j.bbadis.2014.09.007
http://www.eprints.iicb.res.in/2401/ |
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