Dual Functionalized Graphene Oxide Serves as a Carrier for Delivering Oligohistidine- and Biotin-Tagged Biomolecules into Cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Dual Functionalized Graphene Oxide Serves as a Carrier for Delivering Oligohistidine- and Biotin-Tagged Biomolecules into Cells
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| Creator |
Jana, Batakrishna
Mondal, Goutam Biswas, Atanu Chakraborty, Indrani Saha, Abhijit Kurkute, Prashant Ghosh, Surajit |
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| Subject |
Chemistry
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| Description |
A versatile method of dual chemical functionalization of graphene oxide (GO) with Tris-[nitrilotris(acetic acid)] (Tris-NTA) and biotin for cellular delivery of oligohistidine- and biotintagged biomolecules is reported. Orthogonally functionalized GO surfaces with Tris-NTA and
biotin to obtain a dual-functionalized GO (DFGO) are prepared and characterized by various spectroscopic and microscopic techniques. Fluorescence microscopic images reveal that DFGO surfaces are capable of binding oligohistidine-tagged biomolecules/proteins and avidin/
biotin-tagged biomolecules/proteins orthogonally. The
DFGO nanoparticles are noncytotoxic in nature and can
deliver oligohistidine- and biotin-tagged biomolecules
simultaneously into the cell
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2413/1/Macromol._Biosci._2013%2C_13%2C_1478%E2%80%931484.pdf
Jana, Batakrishna and Mondal, Goutam and Biswas, Atanu and Chakraborty, Indrani and Saha, Abhijit and Kurkute, Prashant and Ghosh, Surajit (2013) Dual Functionalized Graphene Oxide Serves as a Carrier for Delivering Oligohistidine- and Biotin-Tagged Biomolecules into Cells. Macromolecular Bioscience, 13. pp. 1478-1484. |
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| Relation |
http://www.eprints.iicb.res.in/2413/
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