Effect of corchorusin-D, a saikosaponin like compound, on B16F10 melanoma cells (in vitro and in vivo)
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Effect of corchorusin-D, a saikosaponin like compound, on B16F10 melanoma cells (in vitro and in vivo)
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| Creator |
Mallick, Sumana
Pal, Bikas Chandra Kumar, Deepak Chatterjee, Nabanita Das, Subhadip Das Saha, Krishna |
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| Subject |
Cancer Biology and Inflammatory Disorder Division
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| Description |
Corchorusin-D (COR-D), isolated from Corchorus acutangulus, was reported to induce
apoptosis in leukemic cells. However, no studies concerning its activity on melanoma
cells have been reported. We have evaluated its in vitro anti-cancer activity on
melanoma cells (B16F10, SK-MEL-28, and A375). The results demonstrate that CORD
showed maximum inhibition of B16F10 cells in vitro. COR-D induced
mitochondrial dysfunction and altered the Bax/Bcl-2 ratio with down regulation of
pro-caspases 9 and activation of caspase 3 in B16F10 cells, triggering intrinsic pathway
of apoptosis. Moreover, it inhibited the in vivo B16F10 tumor growth and increased the
survival rate of mice. Greater number of Annexin V-FITC and propidium iodide (PI)-
positive tumor cells signified that COR-D induced apoptosis in vivo also. The reduction
in tumor growth is well correlated with decreased microvascular density of the tumor
cells in treated mice. In conclusion, this study reveals that COR-D-induced
mitochondrial dysfunction is responsible for the induction of apoptotic cell death
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| Publisher |
Taylor & Francis
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2416/1/Journal_of_Asian_Natural_Products_Research%2C_2013.pdf
Mallick, Sumana and Pal, Bikas Chandra and Kumar, Deepak and Chatterjee, Nabanita and Das, Subhadip and Das Saha, Krishna (2013) Effect of corchorusin-D, a saikosaponin like compound, on B16F10 melanoma cells (in vitro and in vivo). Journal of Asian Natural Products Research, 15 (11). pp. 1197-1203. ISSN 1028-6020 |
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| Relation |
http://dx.doi.org/10.1080/10286020.2013.83745
http://www.eprints.iicb.res.in/2416/ |
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