Reduced Virulence of the Vibrio cholerae fadD Mutant Is Due to Induction of the Extracytoplasmic Stress Response
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Reduced Virulence of the Vibrio cholerae fadD Mutant Is Due to Induction of the Extracytoplasmic Stress Response
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| Creator |
Chatterjee, Epshita
Chowdhury, Rukhsana |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Vibrio cholerae, an important human intestinal pathogen, is responsible for the diarrheal disease cholera. The pathogenesis of V.cholerae is a highly coordinated process that involves diverse regulatory factors. It has recently been demonstrated that disruption of the V. cholerae fadD gene, encoding a long-chain fatty acyl coenzyme A (acyl-CoA) ligase, drastically reduces expression of the major virulence genes and in vivo lethality of this important human pathogen. This effect was due to reduced membrane
localization of the central virulence regulator TcpP. In this study, the reason for the impaired membrane localization of TcpP in the fadD mutant was investigated. We demonstrate that extracytoplasmic stress is induced in the V. cholerae �fadD strain. In response to the extracytoplasmic stress, the integral membrane protease RseP is activated and degrades the membrane-localized
TcpP in the fadD mutant strain. Indeed, disruption of the rseP gene in a fadD mutant background restored membrane localization of TcpP and expression of the downstream virulence genes toxT, ctxA, and tcpA. Increased expression of the �E regulon genes in ethanol-treated wild-type V. cholerae indicated that ethanol exposure could induce an extracytoplasmic stress response in V. cholerae. Ethanol treatment also led to activation of the RseP protease activity and resulted in degradation of membranelocalized
TcpP and subsequent reduction in expression of the virulence genes. Taken together, these results suggest that extracytoplasmic stress response per se reduces virulence of V. cholerae by impairing membrane localization of TcpP
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| Publisher |
American Society for Microbiology
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2431/1/INFECTION_AND_IMMUNITY__Vol._81_(_10)____Pages__3935%2D3941;2013.pdf
Chatterjee, Epshita and Chowdhury, Rukhsana (2013) Reduced Virulence of the Vibrio cholerae fadD Mutant Is Due to Induction of the Extracytoplasmic Stress Response. Infection and Immunity, 81 (10). pp. 3935-3941. |
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| Relation |
http://dx.doi.org/10.1128/IAI.00722-13
http://www.eprints.iicb.res.in/2431/ |
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