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Vaccination with Leishmania Hemoglobin Receptor–Encoding DNA Protects Against Visceral Leishmaniasis

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Vaccination with Leishmania Hemoglobin Receptor–Encoding DNA Protects Against Visceral Leishmaniasis
 
Creator Guha, Rajan
Gupta, Deepika
Rastogi, Ruchir
Vikram, Rajagopal
Krishnamurthy, Ganga
Bimal, Sanjiva
Roy, Syamal
Mukhopadhyay, Amitabha
 
Subject Infectious Diseases and Immunology
 
Description Leishmaniasis is a severe infectious disease. Drugs used for leishmaniasis are very toxic, and no vaccine is available.We found that the hemoglobin receptor (HbR) of Leishmania was conserved across various strains of Leishmania,and anti-HbR antibody could be detected in kala-azar patients’ sera. Our results showed that immunization with HbR-DNA induces complete protection against virulent Leishmania donovani infection in both BALB/c mice and hamsters. Moreover, HbR-DNA immunization stimulated the production of protective cytokines like interferon-g (IFN-g), interleukin-12 (IL-12), and tumor necrosis factor–a (TNF-a) with concomitant down-regulation of diseasepromoting cytokines like IL-10 and IL-4. HbR-DNA vaccination also induced a protective response by generating multifunctional CD4+ and CD8+ T cells. All HbR-DNA–vaccinated hamsters showed sterile protection and survived during an experimental period of 8 months. These findings demonstrate the potential of HbR as a vaccine candidate against visceral leishmaniasis
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2434/1/SCIENCE_TRANSLATIONAL_MEDICINE__Volume__5____Issue__202______Article_Number__202ra121;2013.pdf
Guha, Rajan and Gupta, Deepika and Rastogi, Ruchir and Vikram, Rajagopal and Krishnamurthy, Ganga and Bimal, Sanjiva and Roy, Syamal and Mukhopadhyay, Amitabha (2013) Vaccination with Leishmania Hemoglobin Receptor–Encoding DNA Protects Against Visceral Leishmaniasis. Science Translational Medicine, 5 (202). 01p.-13p..
 
Relation http://www.eprints.iicb.res.in/2434/