Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Non-mulberry Silk Fibroin Biomaterial for Corneal
Regeneration
|
|
| Creator |
Hazra, Sarbani
Nandi, Sudip Naskar, Deboki Guha, Rajdeep Chowdhury, Sushovan Pradhan, Nirparaj Kundu, Subhas C Konar, Aditya |
|
| Subject |
Animal House
|
|
| Description |
Successful repair of a damaged corneal surface is a great challenge and may require the use of a scaffold that supports cell growth and differentiation. Amniotic membrane is currently used for this purpose, in spite of its limitations. A thin transparent silk fibroin film from non-mulberry Antheraea mylitta (Am) has been developed which offers to be a promising alternative. The silk scaffolds provide sufficient rigidity for easy handling, the scaffolds support the sprouting, migration, attachment
and growth of epithelial cells and keratocytes from rat corneal explants; the cells form a cell sheet,
preserve their phenotypes, express cytokeratin3 and vimentin respectively. The films also support growth of limbal stem cell evidenced by expression of ABCG2. The cell growth on the silk film and the amniotic membrane is comparable. The implanted film within the rabbit cornea remains transparent, stable. The clinical examination as well as histology shows absence of any inflammatory response or neovascularization. The corneal surface integrity is maintained; tear formation, intraocular pressure and electroretinography of implanted eyes show no adverse changes. The silk fibroin film from nonmulberry
silk worms may be a worthy candidate for use as a corneal scaffold.
|
|
| Date |
2016
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/2466/1/SCIENTIFIC_REPORTS__Volume__6______Article_Number__21840;2016.pdf
Hazra, Sarbani and Nandi, Sudip and Naskar, Deboki and Guha, Rajdeep and Chowdhury, Sushovan and Pradhan, Nirparaj and Kundu, Subhas C and Konar, Aditya (2016) Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration. Scientific Reports, 6 (21840). 01.-13. |
|
| Relation |
http://dx.doi.org/10.1038/srep21840
http://www.eprints.iicb.res.in/2466/ |
|