Selective Recognition of H3.1K36 Dimethylation/H4K16 Acetylation Facilitates the Regulation of All-trans-retinoic Acid (ATRA)-responsive Genes by Putative Chromatin Reader ZMYND8
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Selective Recognition of H3.1K36 Dimethylation/H4K16
Acetylation Facilitates the Regulation of All-trans-retinoic
Acid (ATRA)-responsive Genes by Putative Chromatin Reader
ZMYND8
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| Creator |
Adhikary, Santanu
Sanyal, Sulagna Basu, Moitri Sengupta, Isha Sen, Sabyasachi Srivastava, Dushyant Kumar Roy, Siddhartha Das, Chandrima |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
ZMYND8 (zinc finger MYND (Myeloid, Nervy and DEAF-1)-
type containing 8), a newly identified component of the transcriptional coregulator network, was found to interact with the Nucleosome Remodeling and Deacetylase (NuRD) complex. Previous reports have shown that ZMYND8 is instrumental in recruiting the NuRD complex to damaged chromatin for repressing transcription and promoting double strand break repair by homologous recombination. However, the mode of transcription regulation byZMYND8has remained elusive. Here, we report that through its specific key residues present in its conserved chromatin-
binding modules,ZMYND8interacts with the selective epigenetic marks H3.1K36Me2/H4K16Ac. Furthermore, ZMYND8
shows a clear preference for canonical histone H3.1 over variant H3.3. Interestingly, ZMYND8 was found to be recruited to several developmental genes, including the all-trans-retinoic acid (ATRA)-responsive ones, through its modified histone-binding ability. Being itself inducible byATRA,this zinc finger transcription factor is involved in modulating other ATRA-inducible genes. We found
that ZMYND8 interacts with transcription initiation-competent RNA polymerase II phosphorylated at Ser-5 in a DNA templatedependent manner and can alter the global gene transcription. Overall, our study identifies that ZMYND8 has CHD4-independent functions in regulating gene expression through its modified histone-binding ability.
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| Publisher |
American Society for Biochemistry and Molecular Biology
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2468/1/THE_JOURNAL_OF_BIOLOGICAL_CHEMISTRY_VOL.291%E2%80%A2No_6%E2%80%A2FEB._5%2C_2016.pdf
Adhikary, Santanu and Sanyal, Sulagna and Basu, Moitri and Sengupta, Isha and Sen, Sabyasachi and Srivastava, Dushyant Kumar and Roy, Siddhartha and Das, Chandrima (2016) Selective Recognition of H3.1K36 Dimethylation/H4K16 Acetylation Facilitates the Regulation of All-trans-retinoic Acid (ATRA)-responsive Genes by Putative Chromatin Reader ZMYND8. The Journal of Biological Chemistry, 291 (6). pp. 2664-2681. |
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| Relation |
http://dx.doi.org/DOI 10.1074/jbc.M115.679985
http://www.eprints.iicb.res.in/2468/ |
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