Binding of the alkaloid aristololactam-β-Dglucoside and daunomycin to human hemoglobin:spectroscopy and calorimetry studies
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Binding of the alkaloid aristololactam-β-Dglucoside
and daunomycin to human hemoglobin:spectroscopy and calorimetry studies
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| Creator |
Das, Abhi
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| Subject |
Chemistry
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| Description |
The interaction of the plant alkaloid aristololactam-β-D-glucoside (ADG) and the anticancer agent daunomycin (DAN)
with human hemoglobin was studied by different spectroscopic and calorimetric methods. The binding affinity values of ADG and DAN, estimated from spectroscopic experiments, were 3.79 × 104 and 6.68 × 104 M−1, respectively. From circular dichroism, 3D fluorescence, and FTIR studies it was observed that, DAN induced stronger conformational changes than ADG in the protein. From synchronous fluorescence spectroscopy results, a pronounced shift in the maximum emission wavelength of tyrosine residues was observed in both cases suggesting that the drugs changed the polarity around tyrosine residues with marginal change around the tryptophan residues. The thermodynamics of the binding
interaction analyzed using microcalorimetry presented single binding events that were exothermic in nature in both cases.The binding was driven by large positive standard molar entropy changes with small favorable enthalpy contributions.Negative heat capacity changes in both cases are correlated to the involvement of significant hydrophobic forces in the complexation process. The affinity of DAN to Hb was higher than that of ADG.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2475/1/Journal_of_Biomolecular_Structure_and_Dynamics;2016.pdf
Das, Abhi (2016) Binding of the alkaloid aristololactam-β-Dglucoside and daunomycin to human hemoglobin:spectroscopy and calorimetry studies. Journal of Biomolecular Structure and Dynamics, 34 (4). pp. 800-813. |
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| Relation |
http://dx.doi.org/10.1080/07391102.2015.1055304
http://www.eprints.iicb.res.in/2475/ |
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