MicroRNA profiling of cisplatinresistant oral squamous cell carcinoma cell lines enriched withcancer-stem-cell-like and epithelial-mesenchymal transition-type features
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
MicroRNA profiling of cisplatinresistant
oral squamous cell carcinoma cell lines enriched withcancer-stem-cell-like and epithelial-mesenchymal transition-type features
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| Creator |
Dey Ghosh, Ruma
Ghuwalewala, Sangeeta Das, Pijush Mandloi, Sapan Alam, Sk Kayum Chakraborty, Jayanta Sarkar, Sajal Chakrabarti, Saikat Panda, Chinmoy Kumar Roychoudhury, Susanta |
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| Subject |
Cancer Biology and Inflammatory Disorder Division
Structural Biology & Bioinformatics |
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| Description |
Oral cancer is of major public health problem in India. Current investigation was aimed to identify
the specific deregulated miRNAs which are responsible for development of resistance phenotype
through regulating their resistance related target gene expression in oral squamous cell carcinoma
(OSCC). Cisplatin-resistant OSCC cell lines were developed from their parental human OSCC cell lines
and subsequently characterised. The resistant cells exhibited enhanced proliferative, clonogenic
capacity with significant up-regulation of P-glycoprotein (ABCB1), c-Myc, survivin, β-catenin and a
putative cancer-stem-like signature with increased expression of CD44, whereas the loss of E-cadherin
signifies induced EMT phenotype. A comparative analysis of miRNA expression profiling in parental
and cisplatin-resistant OSCC cell lines for a selected sets (deregulated miRNAs in head and neck cancer)
revealed resistance specific signature. Moreover, we observed similar expression pattern for these
resistance specific signature miRNAs in neoadjuvant chemotherapy treated and recurrent tumours
compared to those with newly diagnosed primary tumours in patients with OSCC. All these results
revealed that these miRNAs play an important role in the development of cisplatin-resistance mainly
through modulating cancer stem-cell-like and EMT-type properties in OSCC.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2478/1/SCIENTIFIC_REPORTS__Volume__6__Article_Number__23932;2016.pdf
Dey Ghosh, Ruma and Ghuwalewala, Sangeeta and Das, Pijush and Mandloi, Sapan and Alam, Sk Kayum and Chakraborty, Jayanta and Sarkar, Sajal and Chakrabarti, Saikat and Panda, Chinmoy Kumar and Roychoudhury, Susanta (2016) MicroRNA profiling of cisplatinresistant oral squamous cell carcinoma cell lines enriched withcancer-stem-cell-like and epithelial-mesenchymal transition-type features. Scientific Reports, 6 (23932). pp. 1-15. |
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| Relation |
http://dx.doi.org/10.1038/srep23932
http://www.eprints.iicb.res.in/2478/ |
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