A Lipid Based Antigen Delivery System Efficiently Facilitates MHC Class-I Antigen Presentation in Dendritic Cells to Stimulate CD8+ T Cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
A Lipid Based Antigen Delivery System Efficiently Facilitates MHC Class-I Antigen Presentation in Dendritic Cells to Stimulate CD8+ T Cells
|
|
| Creator |
Maji, Mithun
Mazumder, Saumyabrata Bhattacharya, Souparno Choudhury, Somsubhra Thakur Sabur, Abdus Shadab, Md. Shadab Bhattacharya, Pradyot Ali, Nahid |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
The most effective strategy for protection against intracellular infections such as Leishmania is
vaccination with live parasites. Use of recombinant proteins avoids the risks associated with live
vaccines. However, due to low immunogenicity, they fail to trigger T cell responses particularly of CD8+cells requisite for persistent immunity. Previously we showed the importance of protein entrapment in cationic liposomes and MPL as adjuvant for elicitation of CD4+ and CD8+ T cell responses for longterm protection. In this study we investigated the role of cationic liposomes on maturation and antigen presentation capacity of dendritic cells (DCs). We observed that cationic liposomes were taken up very
efficiently by DCs and transported to different cellular sites. DCs activated with liposomal rgp63 led to
efficient presentation of antigen to specific CD4+ and CD8+ T cells. Furthermore, lymphoid CD8+ T cells from liposomal rgp63 immunized mice demonstrated better proliferative ability when co-cultured ex vivo with stimulated DCs. Addition of MPL to vaccine enhanced the antigen presentation by DCs and induced more efficient antigen specific CD8+ T cell responses when compared to free and liposomal ntigen. These liposomal formulations presented to CD8+ T cells through TAP-dependent MHC-I pathway offer new possibilities for a safe subunit vaccine.
|
|
| Date |
2016
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/2512/1/Sci_Reports_2016.pdf
Maji, Mithun and Mazumder, Saumyabrata and Bhattacharya, Souparno and Choudhury, Somsubhra Thakur and Sabur, Abdus and Shadab, Md. Shadab and Bhattacharya, Pradyot and Ali, Nahid (2016) A Lipid Based Antigen Delivery System Efficiently Facilitates MHC Class-I Antigen Presentation in Dendritic Cells to Stimulate CD8+ T Cells. Scientific Reports, 6 (27206). |
|
| Relation |
http://dx.doi.org/10.1038/srep27206
http://www.eprints.iicb.res.in/2512/ |
|