Assessment of SYBR green I dye-based fluorescence assay for screening antimalarial activity of cationic peptides and DNA intercalating agents.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Assessment of SYBR green I dye-based fluorescence assay for screening antimalarial activity of cationic peptides and DNA intercalating agents.
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| Creator |
Bhatia, Rakesh
Gautam, Ankur Gautam, Shailendra K Mehta, Divya Kumar, Vinod Raghava, G.P.S. Varshney, Grish C |
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| Subject |
QR Microbiology
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| Description |
The SYBR green I (SG) dye-based fluorescence assay for screening antimalarial compounds is based on direct quantitation of parasite DNA. We show that DNA-interacting cationic cell-penetrating peptides (CPPs) and intercalating agents compete with SG dye to bind to DNA. Therefore, readouts of this assay, unlike those of the [(3)H]hypoxanthine incorporation assay, for the antimalarial activity of the above DNA binding agents may be erroneous. In the case of CPPs, false readouts can be improved by the removal of excess peptides.
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| Publisher |
American Society for Microbiology
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| Date |
2015-05
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| Type |
Article
PeerReviewed |
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| Relation |
http://aac.asm.org/content/early/2015/02/10/AAC.03266-14.full.pdf
http://crdd.osdd.net/open/1661/ |
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| Identifier |
Bhatia, Rakesh and Gautam, Ankur and Gautam, Shailendra K and Mehta, Divya and Kumar, Vinod and Raghava, G.P.S. and Varshney, Grish C (2015) Assessment of SYBR green I dye-based fluorescence assay for screening antimalarial activity of cationic peptides and DNA intercalating agents. Antimicrobial agents and chemotherapy, 59 (5). pp. 2886-9. ISSN 1098-6596
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