Monoamine oxidase B gene variants associated with attention deficit hyperactivity disorder in the Indo-Caucasoid population from West Bengal
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Monoamine oxidase B gene variants associated with attention deficit hyperactivity disorder in the Indo-Caucasoid population from West Bengal
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| Creator |
Karmakar, Arijit
Maitra, Subhamita Chakraborti, Barnali Verma, Deepak Sinha, Swagata Mohanakumar, Kochupurackal P Rajamma, Usha Mukhopadhyay, Kanchan |
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| Subject |
Cell Biology & Physiology
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| Description |
Attention deficit hyperactivity disorder (ADHD) is characterized by symptoms of inattention, excessive
motor activity and impulsivity detected mostly during childhood. These traits are known to be controlled by
monoamine neurotransmitters, chiefly dopamine, serotonin and norepinephrine. Monoamine oxidase A (MAOA)
and B (MAOB), two isoenzymes bound to the outer membrane of mitochondria, are involved in the degradation of
monoamines and were explored for association with ADHD in different ethnic groups. In the present study, few
exonic as well as intronic MAOB variants were analyzed in ADHD probands (N = 150) and ethnically matched
controls (N = 150) recruited following the Diagnostic and Statistical Manual for Mental Disorders-4th edition
(DSM-IV). Appropriate scales were used for measuring the behavioural attributes. Gene variants were analyzed by
amplification of target sites followed by DNA sequencing and data obtained were analyzed by population based
statistical methods.
Results: Out of 34 variants present in the analyzed sites, only seven functional variants, rs4824562, rs56220155,
rs2283728, rs2283727, rs3027441, rs6324 and rs3027440, were found to be polymorphic. rs2283728 ‘C’ (P = 3.45e-006)
and rs3027440 ‘T’ (P = 0.02) alleles showed higher frequencies in ADHD probands as compared to controls.
rs56220155 ‘A’ (P = 0.04) allele and ‘GA’ (P = 0.04) genotype showed higher frequencies in the male and female
ADHD probands respectively as compared to sex-matched controls. Analysis of pairwise linkage disequilibrium
revealed striking differences between probands and controls. Haplotype analysis revealed significantly higher
occurrence of different haplotypes in the ADHD probands while some haplotypes were detected in the controls
only. Higher scores for conduct problems were found to be associated with rs56220155 ‘A’ (P = 0.05) allele in the
male ADHD probands. Multifactor dimensionality reduction analysis showed independent as well as interactive
effects of polymorphic variants which were more robust in the male probands.
Conclusions: Since all the polymorphic variants analyzed were functional, it may be inferred that MAOB gene
variants are contributing to the etiology of ADHD in the Indo-Caucasoid population from eastern India which
merits further in depth analysis.
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| Publisher |
BioMed Central
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2528/1/BMC_Genetics_(2016)_1792.pdf
Karmakar, Arijit and Maitra, Subhamita and Chakraborti, Barnali and Verma, Deepak and Sinha, Swagata and Mohanakumar, Kochupurackal P and Rajamma, Usha and Mukhopadhyay, Kanchan (2016) Monoamine oxidase B gene variants associated with attention deficit hyperactivity disorder in the Indo-Caucasoid population from West Bengal. BMC Genetics, 17 (92). 01p. -15p.. |
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| Relation |
http://dx.doi.org/10.1186/s12863-016-0401-6
http://www.eprints.iicb.res.in/2528/ |
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