Unraveling the Interaction of Silver Nanoparticles with Mammalian and Bacterial DNA
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Unraveling the Interaction of Silver Nanoparticles with Mammalian and Bacterial DNA
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| Creator |
Pramanik, Srikrishna
Chatterjee, Sabyasachi Saha, Arindam Parukuttyamma, Sujatha Devi Kumar, Gopinatha Suresh |
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| Subject |
Chemistry
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| Description |
The focus of this study was to understand and
unravel the interaction of silver nanoparticles (AgNPs) with
different types of Deoxyribonucleic acid (DNA), mammalian
and bacterial, having different base pair compositions. Binding
of spherical silver nanoparticles (AgNPs) to Calf thymus (CT)
DNA, Escherichia coli (EC) DNA and Micrococcus lysodeikticus
(ML) DNA has been studied to gain insights into their mode
of interaction and specificity. Interaction of AgNPs with
synthetic DNA has also been carried out. On the basis of
absorption, thermal melting, isothermal calorimetry and
viscosity studies, we could establish the mode of binding and
specificity of the synthesized silver nanoparticles with
mammalian and bacterial DNA. Thermal melting (Tm) studies indicated a decrease in the Tm of all the DNAs, confirming
the destabilization of DNA stacks on interaction with AgNPs. Comparative interaction studies with single stranded (ss) and
double stranded (ds) DNAs further confirmed the specificity of the particles toward ds DNA. On the basis of the results we could
confirm that the synthesized AgNPs could be used for selective detection of DNA through their DNA binding mechanism. In
addition, the AgNPs−DNA complexes exhibited distinct differences in the SERS spectra making it an interesting SERS platform
for identifying ds DNA. The optical and physical properties of AgNPs help in differentiating the DNAs of different base pair
compositions through their binding affinity and specificity
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| Publisher |
American Chemical Society
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2529/1/The_Journal_of_Physical_Chemistry_B.pdf
Pramanik, Srikrishna and Chatterjee, Sabyasachi and Saha, Arindam and Parukuttyamma, Sujatha Devi and Kumar, Gopinatha Suresh (2016) Unraveling the Interaction of Silver Nanoparticles with Mammalian and Bacterial DNA. The Journal of Physical Chemistry B, 120. pp. 5313-5324. |
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| Relation |
http://dx.doi.org/10.1021/acs.jpcb.6b01586
http://www.eprints.iicb.res.in/2529/ |
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