Molecular Details of Acetate Binding to a New Diamine Receptor by NMR and FT-IR Analyses
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Molecular Details of Acetate Binding to a New Diamine Receptor by NMR and FT-IR Analyses
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| Creator |
Banerji, Biswadip
Chatterjee, Moumita Pal, Uttam Maiti, Nakul Chandra |
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| Subject |
Chemistry
Structural Biology & Bioinformatics |
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| Description |
Acetate anion plays an important role in several
biochemical functions such as enzyme reaction, antibody response, and action of receptor molecules. This investigation reports the synthesis and molecular details of a unique receptor, 2-amino-N-(2- amino-benzyl)-benzamide (R) that senses selectively acetate via simultaneous involvement of one aromatic amine group and an amide proton of the receptor molecule. Solution-state NMR, steady-state fluorescence, and FT-IR examinations established that
the acetate anion binds to the receptor with 1:1 ratio with high specificity. The binding was stabilized by two H-bond formations between the oxygen atoms of acetate anion and two H atoms, one from amide group and the other from the amine group of the receptor. The binding interaction caused significant changes in the chemical shift of the receptor protons, and the evaluated affinity constant, from the NMR measurements, was found to be 1.87 × 104 M−1. Density functional theory (DFT) analysis further showed a significant rotation of one of the two aromatic rings leading to formation of a 10-member ring involving the acetate anion, amide proton, and the one amine group attached to aromatic ring. The H-bond patterns observed in the crystal structure were significantly changed due to complex formation. However, the changes in the geometrical arrangement in the complex caused a small but significant increase of the fluorescence emission. Acetate geometry and unique positioning of the amide and amine groups of the receptor render the recognition feasible, and DFT analysis estimated ∼30 kJ M−1 stabilization due to 1:1 complexation. Such positioning and geometrical arrangement may make the receptor very specific to bind acetate anion, and as such R became a very relevant molecule in detection and function of the acetate anion present in complex biochemical systems
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| Publisher |
American Chemical Society
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2537/1/The_Journal_of_Physical_Chemistry_A.pdf
Banerji, Biswadip and Chatterjee, Moumita and Pal, Uttam and Maiti, Nakul Chandra (2016) Molecular Details of Acetate Binding to a New Diamine Receptor by NMR and FT-IR Analyses. The Journal of Physical Chemistry A, 120 (15). pp. 2330-2341. |
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| Relation |
http://dx.doi.org/10.1021/acs.jpca.6b01078
http://www.eprints.iicb.res.in/2537/ |
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