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Spectral mapping of 3D multi-cellular tumor spheroids: time-resolved confocal microscopy

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata


Field	Value

Title	Spectral mapping of 3D multi-cellular tumor spheroids: time-resolved confocal microscopy

Creator	Mohapatra, Saswat Nandi, Somen Chowdhury, Rajdeep Das, Gaurav Ghosh, Surajit Bhattacharyya, Kankan

Subject	Chemistry

Description	A tumor-like multi-cellular spheroid (3D) differs from a 2D cell in a number of ways. This is demonstrated using time resolved confocal microscopy. Two different tumor spheroids – HeLa (cervical cancer) and A549 (lung cancer) – are studied using 3 different fluorescent dyes – C153 (non-covalent), CPM (covalent) and doxorubicin (non-covalent, anti-cancer drug). The pattern of localization of these three fluorescent probes in the 3D tumor cell exhibits significant differences from that in the conventional 2D cells. For both the cells (HeLa and A549), the total uptake of doxorubicin in the 3D cell is much lower than that in the 2D cell. The uptake of doxorubicin molecules in the A549 spheroid is significantly different compared to the HeLa spheroid. The local polarity (i.e. emission maxima) and solvation dynamics in the 3D tumor cell differ from those in 2D cells. The covalent probe CPM exhibits intermittent fluorescence oscillations in the 1–2 s time scale. This is attributed to redox processes. These results may provide new insights into 3D tumors.

Date	2016

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://www.eprints.iicb.res.in/2547/1/Phys._Chem._Chem._Phys.%2C_2016%2C_18%2C_18381%2D%2D18390.pdf Mohapatra, Saswat and Nandi, Somen and Chowdhury, Rajdeep and Das, Gaurav and Ghosh, Surajit and Bhattacharyya, Kankan (2016) Spectral mapping of 3D multi-cellular tumor spheroids: time-resolved confocal microscopy. Phys. Chem. Chem. Phys, 18. pp. 18381-18390.

Relation	http://dx.doi.org/10.1039/c6cp02748b http://www.eprints.iicb.res.in/2547/