Target-dependent biogenesis of cognate microRNAs in human cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Target-dependent biogenesis of cognate microRNAs in human cells
|
|
| Creator |
Bose, Mainak
Bhattacharyya, Suvendra Nath |
|
| Subject |
Molecular & Human Genetics
|
|
| Description |
Extensive research has established how miRNAs regulate target mRNAs by translation
repression and/or endonucleolytic degradation in metazoans. However, information related to
the effect of target mRNA on biogenesis and stability of corresponding miRNAs in animals is
limited. Here we report regulated biogenesis of cognate miRNAs by their target mRNAs.
Enhanced pre-miRNA processing by AGO-associated DICER1 contributes to this increased
miRNP formation. The processed miRNAs are loaded onto AGO2 to form functionally
competent miRISCs both in vivo and also in a cell-free in vitro system. Thus, we identify an
additional layer of posttranscriptional regulation that helps the cell to maintain requisite levels
of mature forms of respective miRNAs by modulating their processing in a target-dependent
manner, a process happening for miR-122 during stress reversal in human hepatic cells
|
|
| Date |
2016
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/2548/1/NATURE_COMMUNICATIONS.pdf
Bose, Mainak and Bhattacharyya, Suvendra Nath (2016) Target-dependent biogenesis of cognate microRNAs in human cells. Nature Communication, 7. |
|
| Relation |
http://dx.doi.org/10.1038/ncomms12200
http://www.eprints.iicb.res.in/2548/ |
|