Spectroscopic and microcalorimetric studies on the molecular binding of food colorant acid red 27 with deoxyribonucleic acid
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Spectroscopic and microcalorimetric studies on the molecular binding of food colorant acid red 27 with deoxyribonucleic acid
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| Creator |
Basu, Anirban
Kumar, Gopinatha Suresh |
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| Subject |
Chemistry
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| Description |
Interaction of the food colorant acid red 27 with double stranded DNA was investigated using spectroscopic and
calorimetric methods. Absorbance and fluorescence studies suggested an intimate binding interaction between
the dye and DNA. The quantum efficiency value testified an effective energy transfer from the DNA base pairs to
the dye molecules. Minor groove displacement assay with Hoechst 33258 revealed that the binding occurs in the minor
groove of DNA. Circular dichroism studies revealed that acid red 27 induces moderate conformational perturbations
in DNA. Results of calorimetric studies suggested that the complexation process was driven largely by positive
entropic contribution with a smaller favorable enthalpy contribution. The equilibrium constant of the binding was
calculated to be (3.04 ± 0.09) × 104M�1 at 298.15 K. Negative heat capacity value along with the enthalpy–entropy
compensation phenomenon established the involvement of dominant hydrophobic forces in the binding process.
Differential scanning calorimetry studies presented evidence for an increased thermal stability of DNA on binding of acid red 27
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| Publisher |
John Wiley & Sons
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2552/1/J._Mol._Recognit._2016.pdf
Basu, Anirban and Kumar, Gopinatha Suresh (2016) Spectroscopic and microcalorimetric studies on the molecular binding of food colorant acid red 27 with deoxyribonucleic acid. Journal of Molecular Recognition, 29. pp. 363-369. ISSN 0952-3499 |
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| Relation |
http://dx.doi.org/10.1002/jmr.2536
http://www.eprints.iicb.res.in/2552/ |
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