CSIR Central

A web-based resource for designing therapeutics against Ebola Virus.

IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh


Field	Value

Title	A web-based resource for designing therapeutics against Ebola Virus.

Creator	Dhanda, Sandeep Kumar Chaudhary, Kumardeep Gupta, Sudheer Brahmachari, Samir Kumar Raghava, G.P.S.

Subject	QR Microbiology

Description	In this study, we describe a web-based resource, developed for assisting the scientific community in designing an effective therapeutics against the Ebola virus. Firstly, we predicted and identified experimentally validated epitopes in each of the antigens/proteins of the five known ebolaviruses. Secondly, we generated all the possible overlapping 9mer peptides from the proteins of ebolaviruses. Thirdly, conserved peptides across all the five ebolaviruses (four human pathogenic species) with no identical sequence in the human proteome, based on 1000 Genomes project, were identified. Finally, we identified peptide or epitope-based vaccine candidates that could activate both the B- and T-cell arms of the immune system. In addition, we also identified efficacious siRNAs against the mRNA transcriptome (absent in human transcriptome) of all the five ebolaviruses. It was observed that three species can potentially be targeted by a single siRNA (19mer) and 75 siRNAs can potentially target at least two species. A web server, EbolaVCR, has been developed that incorporates all the above information and useful computational tools (http://crdd.osdd.net/oscadd/ebola/).

Publisher	NPG

Date	2016

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://crdd.osdd.net/open/1862/1/20.pdf Dhanda, Sandeep Kumar and Chaudhary, Kumardeep and Gupta, Sudheer and Brahmachari, Samir Kumar and Raghava, G.P.S. (2016) A web-based resource for designing therapeutics against Ebola Virus. Scientific reports, 6. p. 24782. ISSN 2045-2322

Relation	http://www.nature.com/articles/srep24782 http://crdd.osdd.net/open/1862/