Noninvasive Diagnosis of Visceral Leishmaniasis:Development and Evaluation of Two Urine-Based Immunoassays for Detection of Leishmania donovani Infection in India
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Noninvasive Diagnosis of Visceral
Leishmaniasis:Development and Evaluation of Two Urine-Based Immunoassays for Detection of Leishmania donovani Infection in India
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| Creator |
Ejazi, Sarfaraz Ahmad
Bhattacharya, Pradyot Bakhteya, Md. Asjad Karim Mumtaz, Aquil Ahmad Pandey, Krishna Ravi Das, Vidya Nand Das, Pradeep Rahaman, Mehebubar Goswami, Rama Prosad Ali, Nahid |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Visceral leishmaniasis (VL), one of the most prevalent parasitic diseasesin the developing world causes serious health concerns. Post kala-azar dermal leishmaniasis (PKDL) is a skin disease which occurs after treatment as a sequel to VL. Parasitological diagnosis involves invasive tissue aspiration which is tedious and painful. Commercially available immunochromatographic rapid diagnostic test such as rK39-RDT is used for field diagnosis of VL, detects antibodiesin serum samples. Urine sample is however, much easier in
collection,storage and handling than serum and would be a better alternative where collection of tissue aspirate or blood is impractical. In this study, we have developed and evaluated the performance of two urine-based diagnostic assays, ELISA and dipstick test, and
compared the results with serologicalrK39-RDT. Our study shows the capability of urinebased tests in detecting anti-Leishmania antibodies effectively for both VL and PKDL diagnosis. The ability of dipstick test to demonstrate negative results after six months in
90% of the VL cases after treatment could be useful as a test of clinical cure. Urine-based
tests can therefore replace the need for invasive practices and ensure better diagnosis
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| Publisher |
Public Library of Science
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2563/1/PLoS_Negl_Trop_Dis_Sarfaraz_2016.PDF
Ejazi, Sarfaraz Ahmad and Bhattacharya, Pradyot and Bakhteya, Md. Asjad Karim and Mumtaz, Aquil Ahmad and Pandey, Krishna and Ravi Das, Vidya Nand and Das, Pradeep and Rahaman, Mehebubar and Goswami, Rama Prosad and Ali, Nahid (2016) Noninvasive Diagnosis of Visceral Leishmaniasis:Development and Evaluation of Two Urine-Based Immunoassays for Detection of Leishmania donovani Infection in India. PLOS Neglected Tropical Diseases. |
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| Relation |
http://dx.doi.org/10.1371/journal.pntd.0005035
http://www.eprints.iicb.res.in/2563/ |
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