Synthesis, characterization and in vitro cytotoxicity assessment of hydroxyapatite from different bioresources for tissue engineering application
IR@CMERI: CSIR- Central Mechanical Engineering Research Institute (CMERI), Durgapur
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| Title |
Synthesis, characterization and in vitro cytotoxicity assessment of hydroxyapatite from different bioresources for tissue engineering application
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| Creator |
Mondal, Sudip
Bardhan, Rajashree Mondal, Biswanath Dey, Apurba Mukhopadhyay, Sudit S. Roy, Syamal Guha, Rajan Roy, Koushik |
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| Subject |
Tissue engineering
Biomaterials |
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| Description |
In the present study, hydroxyapatite (HAp) is synthesized from different biosources like eggshell, fish
scale and bovine bone in a cost effective and ecofriendly way. HAp materials were synthesized from eggshell by
wet precipitation method whereas thermal decomposition method was applied in case of fish scale and bovine bone.
The phase purity and crystallinity of different calcined HAp powder were determined by XRD and FTIR analyses.
The thermogravimetric analysis was carried out to show thermal stability of HAp powder. Average grain sizes of
sintered samples were in submicron range. The morphology of the powders were observed under scanning electron
microscopy (SEM). The dried powders were wet ball milled for several hours and surfactants like Triton-X small
fillers (2 / 4 mm long rod-shaped) were made for in vitro testing. In order to verify the biocompatibility of HAp
powders, cytotoxicity evaluation was carried out in RAW macrophage like cell line media for an incubation period
of 72 h. The cell attachment studies on HAp compacts show an excellent affinity between cells and compact surface.
These results proved high biocompatibility of HAp powders obtained fromdifferent biosources for tissue engineering
applications.
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| Publisher |
Indian Academy of Sciences
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| Date |
2012-08
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| Type |
Article
PeerReviewed |
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| Identifier |
Mondal, Sudip and Bardhan, Rajashree and Mondal, Biswanath and Dey, Apurba and Mukhopadhyay, Sudit S. and Roy, Syamal and Guha, Rajan and Roy, Koushik (2012) Synthesis, characterization and in vitro cytotoxicity assessment of hydroxyapatite from different bioresources for tissue engineering application. Bulletin of Materials Science , 35 (4). pp. 683-691.
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| Relation |
http://cmeri.csircentral.net/118/
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