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Enzymatic Hydrolysis of Water Hyacinth Substrate by Cellulase, Xylanase and � Glucosidase: Experiments and Optimization

IR@CMERI: CSIR- Central Mechanical Engineering Research Institute (CMERI), Durgapur

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Title Enzymatic Hydrolysis of Water Hyacinth Substrate by Cellulase, Xylanase and � Glucosidase: Experiments and Optimization
 
Creator Ganguly, A.
Gupta, A.
Das, S.
Dey, A.
Chatterjee, P.K.
 
Subject Bioethanol
 
Description Bioethanol is a renewable energy source produced from fermentation of sugar released by saccharification of lignocellulosic materials. Pretreatment followed by enzymatic hydrolysis and fermentation has been reported as a very effective process for conversion of lignocellulosics to bioethanol. The present study investigates the effect of enzymatic hydrolysis using different enzymes like cellulase, xylanase and � glucosidase on alkali, acid and untreated water hyacinth and then optimizing the enzyme concentration. The study of the interaction among the three enzymes has been carried out to achieve the maximum total reducing sugar as well as xylose yield. The optimized predicted results of the influencing parameters for maximum yield of total reducing sugar are cellulase concentration of 15 U/g, xylanase concentration of 300 U/g and �-glucosidase concentration of 59.68 U/g, with a value of 431.421 mg/g and a desirability of 0.996.
 
Publisher American Scientific Publishers
 
Date 2012
 
Type Article
PeerReviewed
 
Identifier Ganguly, A. and Gupta, A. and Das, S. and Dey, A. and Chatterjee, P.K. (2012) Enzymatic Hydrolysis of Water Hyacinth Substrate by Cellulase, Xylanase and � Glucosidase: Experiments and Optimization. Journal of Biobased Materials and Bioenergy, 6 (3). pp. 1-9. ISSN 1556-6560
 
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