Characterization and optimization of production of exopolysaccharide from Chlamydomonas reinhardtii.
IR@NEERI: National Environment Engineering Research Institute
View Archive Info| Field | Value | |
| Title |
Characterization and optimization of production of exopolysaccharide from Chlamydomonas reinhardtii.
|
|
| Creator |
Bafana, Amit
|
|
| Subject |
Environmental Biotechnology
Biochemistry |
|
| Description |
Chlamydomonas reinhardtii strain RAC was isolated based on its ability to secrete large amount of exopolysaccharide (EPS). The purified EPS had a molecular weight of 2.25�10(5)Da, and showed fibrillar structure with surfaces having sheet-like appearance. Chemical analysis showed the presence of galacturonic acid, ribose, arabinose, xylose, glucose, galactose and rhamnose sugars. The production of EPS was optimized by the classical one-at-a-time approach and Plackett-Burman design, followed by response surface methodology. The resulting response surface model was statistically significant (p<0.5) and predicted maximum EPS production of 628 mg/L. The optimum production medium consisted of CaCl2 - 74, NaNO3 - 422, K2HPO4 - 10 and MgSO4 - 200mg/L with a pH 7. The EPS showed significant antioxidant activity, which can have several industrial applications. This is the first report on characterization and production of EPS from a Chlamydomonas strain isolated from India. Its differences from the earlier reported EPS are discussed.
|
|
| Publisher |
Elsevier
|
|
| Date |
2013-06-20
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://neeri.csircentral.net/310/1/published.pdf
Bafana, Amit (2013) Characterization and optimization of production of exopolysaccharide from Chlamydomonas reinhardtii. Carbohydrate polymers, 95 (2). pp. 746-752. ISSN 1879-1344 |
|
| Relation |
http://www.sciencedirect.com/science/article/pii/S0144861713001549
http://neeri.csircentral.net/310/ |
|