An integrated genomic and proteomic approach to identify signatures of endosulfan exposure in hepatocellular carcinoma cells
IR@NEERI: National Environment Engineering Research Institute
View Archive Info| Field | Value | |
| Title |
An integrated genomic and proteomic approach to identify signatures of endosulfan exposure in hepatocellular carcinoma cells
|
|
| Creator |
Gandhi, Deepa
Tarale, Prashant Naoghare, Pravin Bafana, Amit Krishnamurthi, Kannan Arrigo, Patrizio Saravana Devi, S |
|
| Subject |
Environmental Health
|
|
| Description |
Present study reports the identi fication of genomic and proteomic signatures of endosulfan exposure in hepatocellular carcinoma cells (HepG2). HepG2 cells were exposed to sublethal concentration (15 μM) of endosulfan for
24 h. DNA microarray and MALDI –TOF–MS analyses revealed that endosulfan induced signi ficant alterations in
the expression level of genes and proteins involved in multiple cellular pathways (apoptosis, transcription, immune/in flammatory response, carbohydrate metabolism, etc.). Furthermore, downregulation of PHLDA gene,
upregulation of ACIN1 protein and caspase-3 activation in exposed cells indicated that endosulfan can trigger apoptotic cascade in hepatocellular carcinoma cells. In total 135 transcripts and 19 proteins were differentially
expressed. This study presents an integrated approach to identify the alteration of biological/cellular pathways
in HepG2 cells upon endosulfan exposure
|
|
| Publisher |
Elsevier
|
|
| Date |
2015-11
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://neeri.csircentral.net/572/1/Endosulfan%20HepG2%20published.pdf
Gandhi, Deepa and Tarale, Prashant and Naoghare, Pravin and Bafana, Amit and Krishnamurthi, Kannan and Arrigo, Patrizio and Saravana Devi, S (2015) An integrated genomic and proteomic approach to identify signatures of endosulfan exposure in hepatocellular carcinoma cells. Pesticide Biochemistry and Physiology, 125. pp. 8-16. ISSN 0048-3575 |
|
| Relation |
http://www.sciencedirect.com/science/article/pii/S0048357515300080
http://neeri.csircentral.net/572/ |
|