Assessment of virulence potential of uncharacterized Enterococcus faecalis strains using pan genomic approach – Identification of pathogen–specific and habitatspecific genes
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Assessment of virulence potential of uncharacterized Enterococcus faecalis strains using pan genomic
approach – Identification of pathogen–specific and habitatspecific genes
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| Creator |
Bakshi, Utpal
Sarkar, Munmun Paul, Sandip Dutta, Chitra |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
Enterococcus faecalis, a leading nosocomial pathogen and yet a prominent member of gut microbiome,
lacks clear demarcation between pathogenic and non-pathogenic strains at genome level. Here we
present the comparative genome analysis of 36 E. faecalis strains with different pathogenic features
and from different body-habitats. This study begins by addressing the genome dynamics, which
shows that the pan-genome of E. faecalis is still open, though the core genome is nearly saturated. We
identified eight uncharacterized strains as potential pathogens on the basis of their co-segregation
with reported pathogens in gene presence-absence matrix and Pathogenicity Island (PAI) distribution.
A ~7.4 kb genomic-cassette, which is itself a part of PAI, is found to exist in all reported and potential
pathogens, but not in commensals and other uncharacterized strains. This region encodes four genes
and among them, products of two hypothetical genes are predicted to be intrinsically disordered that
may serve as novel targets for therapeutic measures. Exclusive existence of 215, 129, 4 and 1 genes
in the blood, gastrointestinal tract, urogenital tract, oral cavity and lymph node derived E. faecalis
genomes respectively suggests possible employment of distinct habitat-specific genetic strategies in
the adaptation of E. faecalis in human host
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| Date |
2016-12-07
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2604/1/Scientific_Reports__638648.pdf
Bakshi, Utpal and Sarkar, Munmun and Paul, Sandip and Dutta, Chitra (2016) Assessment of virulence potential of uncharacterized Enterococcus faecalis strains using pan genomic approach – Identification of pathogen–specific and habitatspecific genes. Scientific Reports, 6. p. 38648. |
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| Relation |
http://dx.doi.org/10.1038/srep38648
http://www.eprints.iicb.res.in/2604/ |
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