α‑Cyclodextrin Interacts Close to Vinblastine Site of Tubulin and Delivers Curcumin Preferentially to the Tubulin Surface of Cancer Cell
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
α‑Cyclodextrin Interacts Close to Vinblastine Site of Tubulin and Delivers Curcumin Preferentially to the Tubulin Surface of Cancer Cell
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| Creator |
Jana, Batakrishna
Mohapatra, Saswat Mondal, Prasenjit Barman, Surajit Pradhan, Krishnangsu Saha, Abhijit Ghosh, Surajit |
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| Subject |
Chemistry
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| Description |
Tubulin is the key cytoskeleton component, which plays
a crucial role in eukaryotic cell division. Many anticancer drugs have been developed targeting the tubulin surface. Recently, it has been shown that few polyhydroxy carbohydrates perturb tubulin polymerization.
Cyclodextrin (CD), a polyhydroxy carbohydrate, has been
extensively used as the delivery vehicle for delivery of hydrophobic drugs to the cancer cell. However, interaction of CD with intracellular components has not been addressed before. In this Article, we have shown for the first time that α-CD interacts with tubulin close to the
vinblastine site using molecular docking and Förster resonance energy transfer (FRET) experiment. In addition, we have shown that α-CD binds with intracellular tubulin/microtubule. It delivers a high amount
of curcumin onto the cancer cell, which causes severe disruption of intracellular microtubules. Finally, we have shown that the inclusion complex of α-CD and curcumin (CCC) preferentially enters into the human lung cancer cell (A549) as compared to the normal lung fibroblast cell (WI38), causes apoptotic death, activates tumor suppressor protein (p53) and cyclin-dependent kinase inhibitor 1 (p21), and inhibits 3D spheroid growth of cancer cell
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| Date |
2016-05
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2610/1/ACS_Applied_Materials_%26_Interfaces_pdf.pdf
Jana, Batakrishna and Mohapatra, Saswat and Mondal, Prasenjit and Barman, Surajit and Pradhan, Krishnangsu and Saha, Abhijit and Ghosh, Surajit (2016) α‑Cyclodextrin Interacts Close to Vinblastine Site of Tubulin and Delivers Curcumin Preferentially to the Tubulin Surface of Cancer Cell. ACS Applied Materials & Interfaces, 8. pp. 13793-13803. |
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| Relation |
http://dx.doi.org/10.1021/acsami.6b03474
http://www.eprints.iicb.res.in/2610/ |
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