Polymer-fabricated synthesis of cerium oxide nanoparticles and applications as a green catalyst towards multicomponent transformation with size-dependent activity studies
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Polymer-fabricated synthesis of cerium oxide
nanoparticles and applications as a green catalyst
towards multicomponent transformation with size-dependent activity studies
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| Creator |
Sil, Pallabi
Paul, Simanta Sarani Silvio, Eva Di Travaglini-Allocatellib, Carlo Chattopadhyay, Krishnananda |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
In this paper, we have studied the equilibrium unfolding transitions of cytochrome c from Pseudomonas
aeruginosa (cytc551), a small bacterial protein. Similar to eukaryotic cytochrome c, cytc551 folds sequentially,
although significant differences exist in the order of folding units (foldons). There are two regions of cytc551
(N-terminal helix with residue number 3 to 10 and the loop 2 region containing residues 34 to 45), in which no
foldon unit could be assigned. In addition, the helix containing the Cys-X-X-Cys-His motif, adjacent to the
N-terminal helix (residue number 3 to 10), shows unexplained ultra-fast collapse. To obtain further insights, we
have studied cytc551 site-directed mutants using fluorescence correlation spectroscopy (FCS) and molecular
dynamics simulation. We have found out that cytc551 unfolds through the formation of a fluorescently dark
intermediate state and the amplitude of the dark component depends on the position of labeling. We have
utilized this position dependence to propose a shape change model during the unfolding of cytc551. The
present results show that the N-terminal helix remains in a collapsed position even in the completely unfolded
state and this helix may act as a rigid support to guide the folding of its adjacent helix. This rigid support may
be responsible for the ultra-fast collapse of the adjacent helix region, which occurs during the initial events of
folding. The present results also show that the C-terminal end of loop 2 traverses a large distance during
unfolding compared to the N-terminal end, which justifies the observed flexibility of the loop 2 region
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| Date |
2016-08-10
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2621/1/Phys._Chem._Chem._Phys._2016.pdf
Sil, Pallabi and Paul, Simanta Sarani and Silvio, Eva Di and Travaglini-Allocatellib, Carlo and Chattopadhyay, Krishnananda (2016) Polymer-fabricated synthesis of cerium oxide nanoparticles and applications as a green catalyst towards multicomponent transformation with size-dependent activity studies. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 18 (35). pp. 24537-24548. |
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| Relation |
http://dx.doi.org/10.1039/c6cp04819f
http://www.eprints.iicb.res.in/2621/ |
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