Self-assembled monolayer for low density lipoprotein detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Self-assembled monolayer for low density
lipoprotein detection
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| Creator |
Matharu, Zimple
Arya, Sunil K. Sumana, G. Gupta, Vinay Malhotra, B. D. |
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| Subject |
Biochemistry & Molecular Biology
Biology Biophysics |
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| Description |
Heparin (HEP) has been covalently immobilized onto 4-aminothiophenol (ATIP) self-assembled monolayer (SAM) deposited onto gold (Au)-coated glass plate for low density lipoprotein (LDL) detection. The HEP/ATP/Au and LDL/ HEP/ATP/Au electrodes have been characterized using cyclic voltammetry (CV) and scanning electron microscopy (SEM). Surface plasmon resonance (SPIR) measurements reveal that HEP/ATP/Au electrode is sensitive to detection of the LDL in the range 0.03 mu M (10mg/dl)-0.39 mu M (130mg/dl). The values of association and dissociation rate constants in the association phase calculated by kinetic analysis have been found to be k(a) = 9.67 X 10(1) M(-1) s(-1) and k(d) = 2.64 X 10(-4) s(-1).
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| Publisher |
John Wiley and Sons
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/2084/1/229.pdf
Matharu, Zimple and Arya, Sunil K. and Sumana, G. and Gupta, Vinay and Malhotra, B. D. (2008) Self-assembled monolayer for low density lipoprotein detection. Journal of Molecular Recognition , 21 (6). 419-424 . ISSN 1099-1352 |
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| Relation |
http://npl.csircentral.net/2084/
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