Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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Title |
Self-assembled monolayer for toxicant detection
using nucleic acid sensor based on surface plasmon
resonance technique
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Creator |
Solanki, Pratima R.
Prabhakar, Nirmal Pandey, M. K. Malhotra, B. D. |
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Subject |
Engineering
Nanoscience/ Nanotechnology |
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Description |
Double stranded calf thymus deoxyribonucleic acid (dsCT-DNA) has been covalently immobilized onto self-assembled monolayer (SAM) of beta-merceptoethanol (MCE) on gold substrates via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry for fabrication of the surface plasmon resonance (SPR) based biosensing device. The dsCT-DNA-MCE-SAM/Au bioelectrode has been characterized using electrochemical impedance spectroscopy, cyclic voltammetry, contact angle measurements and atomic force microscopy, respectively. This biosensing device has been utilized for detection of cypermethrin (0.0005 ppm) using electrochemical and SPR techniques, respectively. The mechanism of interaction of cypermethrin with dsCT-DNA immobilized onto MCE-SAM has been proposed.
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Publisher |
Springer Verlag
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Date |
2008
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://npl.csircentral.net/2085/1/230.pdf
Solanki, Pratima R. and Prabhakar, Nirmal and Pandey, M. K. and Malhotra, B. D. (2008) Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique. Biomedical Microdevices , 10 (5). 757-767 . ISSN 1572-8781 |
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Relation |
http://npl.csircentral.net/2085/
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