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Zinc oxide-chitosan nanobiocomposite for urea sensor

IR@NPL: CSIR-National Physical Laboratory, New Delhi

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Title Zinc oxide-chitosan nanobiocomposite for urea sensor
 
Creator Solanki, Pratima R.
Kaushik, Ajeet
Ansari, Anees A.
Sumana, G.
Malhotra, B. D.
 
Subject Applied Physics/Condensed Matter
Physics
 
Description Zinc oxide (ZnO)-chitosan (CH) nanobiocomposite film onto indium-tin-oxide (ITO) coated glass has been used to immobilize urease (Urs) and glutamate dehydrogenase (GLDH) for urea detection. The presence of ZnO nanoparticles in CH results in its increased surface area and enhanced electron transfer kinetics. The Urs-GLDH/CH-ZnO/ITO bioelectrode characterized using electrochemical, Fourier transform infrared, and scanning electron microscopy studies exhibit linearity of 5-100 mg/dl, detection limit of 3 mg/dl, response time of 10 s, reproducibility as 20 times, and shelf life of 3 months. The low Michaelis-Menten constant (K-m) value (4.92 mg/dl) indicates enhanced affinity of enzyme with nanobiocomposite
 
Publisher American Institute of Physics
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://npl.csircentral.net/2087/1/294.pdf
Solanki, Pratima R. and Kaushik, Ajeet and Ansari, Anees A. and Sumana, G. and Malhotra, B. D. (2008) Zinc oxide-chitosan nanobiocomposite for urea sensor. Applied Physics Letters, 93 (16). 163903-1-163903-3. ISSN 1077-3118
 
Relation http://npl.csircentral.net/2087/