Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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Title |
Zinc oxide nanoparticles-chitosan composite film for
cholesterol biosensor
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Creator |
Khan, Raju
Kaushik, Ajeet Solanki, Pratima R. Ansari, Anees A. Pandey, Manoj K. Malhotra, B. D. |
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Subject |
Chemistry
Analytical Chemistry |
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Description |
Zinc oxide nanoparticles (NanoZnO) uniformly dispersed in chitosan (CHIT) have been used to fabricate a hybrid nanocomposite film onto indium-tin-oxide (ITO) glass plate. Cholesterol oxidase (ChOx) has been immobilized onto this NanoZnO-CHIT composite film using physiosorption technique. Both NanoZnO-CHIT/ITO electrode and ChOx/NanoZnO-CHIT/ITO bioelectrode have been characterized using Fourier transform-infrared (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) techniques, respectively. The ChOx/NanoZnO-CHIT/ITO bioelectrode exhibits linearity from 5 to 300 mg dl(-1) of cholesterol with detection limit as 5 mg dl(-1), sensitivity as 1.41 x 10(-4) A mg dl(-1) and the value of Michaelis-Menten constant (K-m) as 8.63 mg dl(-1). This cholesterol biosensor can be used to estimate cholesterol in serum samples.
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Publisher |
Elsevier
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Date |
2008
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://npl.csircentral.net/2088/1/293.pdf
Khan, Raju and Kaushik, Ajeet and Solanki, Pratima R. and Ansari, Anees A. and Pandey, Manoj K. and Malhotra, B. D. (2008) Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor. Analytica Chimica Acta , 616 (2). pp. 207-213. ISSN 0003-2670 |
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Relation |
http://npl.csircentral.net/2088/
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