Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies
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| Creator |
Gaonkar, Raghuvir H
Ganguly, Soumya Dewanjee, Saikat Sinha, Samarendu Gupta, Amit Ganguly, Shantanu Chattopadhyay, Dipankar Chatterjee Debnath, Mita |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Garcinol (GAR) is a naturally occurring polyisoprenylated phenolic compound. It has been recently
investigated for its biological activities such as antioxidant, anti-inflammatory, anti ulcer, and
antiproliferative effect on a wide range of human cancer cell lines. Though the outcomes are very
promising, its extreme insolubility in water remains the main obstacle for its clinical application. Herein
we report the formulation of GAR entrapped PLGA nanoparticles by nanoprecipitation method using
vitamin E TPGS as an emulsifier. The nanoparticles were characterized for size, surface morphology,
surface charge, encapsulation efficiency and in vitro drug release kinetics. The MTT assay depicted a
high amount of cytotoxicity of GAR-NPs in B16F10, HepG2 and KB cells. A considerable amount of cell
apoptosis was observed in B16f10 and KB cell lines. In vivo cellular uptake of fluorescent NPs on B16F10
cells was also investigated. Finally the GAR loaded NPs were radiolabeled with technetium-99m with
>95% labeling efficiency and administered to B16F10 melanoma tumor bearing mice to investigate the
in vivo deposition at the tumor site by biodistribution and scintigraphic imaging study. In vitro cellular
uptake studies and biological evaluation confirm the efficacy of the formulation for cancer treatment
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| Date |
2017-04-03
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2636/1/Scientific_Reports.pdf
Gaonkar, Raghuvir H and Ganguly, Soumya and Dewanjee, Saikat and Sinha, Samarendu and Gupta, Amit and Ganguly, Shantanu and Chattopadhyay, Dipankar and Chatterjee Debnath, Mita (2017) Garcinol loaded vitamin E TPGS emulsified PLGA nanoparticles: preparation, physicochemical characterization, in vitro and in vivo studies. Scientific Reports, 7 (530). 01p. -14p.. |
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| Relation |
http://dx.doi.org/10.1038/s41598-017-00696-6
http://www.eprints.iicb.res.in/2636/ |
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