Antibody conjugated graphene nanocomposites for pathogen detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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| Title |
Antibody conjugated graphene nanocomposites for pathogen detection
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| Creator |
Sign, Chandan
Sumana, Gajjala |
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| Subject |
Electronics and Electrical Engineering
Nanoscience/ Nanotechnology |
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| Description |
Graphene oxide (GO), due to its excellent electrochemical properties and large surface area, known to be highly suitable material for biosensing application. Here, we report in situ synthesis of silver nanopaticles (AgNPs) onto the GO sheets for the electrochemical detection of Salmonella typhimurium (S. typhimurium). The GO-AgNPs composites have been deposited onto the indium tin oxide (ITO) coated glass substrate by the electrophoretic deposition technique. Carbodiimide coupling (EDC-NHS) has been used for the immobilization of antibodies of Salmonella typhimurium (anti-S. typhimurium) for detection of S. typhimurium. The electron microscopy and UV-visible studies reveal successful synthesis GO-AgNPs composites while FT-IR studies suggest the proper immobilization of anti-S. typhi. The cyclic voltammetry (CV) has been utilized for detection using anti-S. typhi/GOAgNPs/ITO based immunoelectrode as a function of S. typhimurium concentration. The fabricated immunosensor shows improved sensitivity of 33.04 mu ACFU-1mLcm-2 in a wide detection range of 10(1) to 10(6) CFUmL(-1). This immunosensor may be utilized for the detection of other food borne pathogens like aflatoxin and E. coli also.
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| Publisher |
IOP Science
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| Date |
2016
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| Type |
Book
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/2145/1/Antibody%20conjugated.pdf
Sign, Chandan and Sumana, Gajjala (2016) Antibody conjugated graphene nanocomposites for pathogen detection. Journal of Physics Conference Series , 704 . IOP Science, Kyushu Inst Technol, Fukuoka, JAPAN. |
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| Relation |
http://npl.csircentral.net/2145/
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