Role of active site residues in catalytic activities of heme based adenylate cyclase from Leishmania major
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Role of active site residues in catalytic activities of heme
based adenylate cyclase from Leishmania major
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| Creator |
Roy, Jayasree
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| Subject |
Structural Biology & Bioinformatics
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| Description |
Leishmania promastigotes inhabit in the midgut of a sandfly, where they are densely packed together and the environment of these promastigotes is then likely to
become hypoxic or even anoxic. Interestingly, Leishmania can survive in low oxygen concentration, which is an exception from Trypanosoma brucei. Although some
transcriptional regulators (HIF genes) in mammals help to response in adaptive responses during hypoxia, Leishmania lacks hypoxic inducible factor (HIF) as well as
transcriptional regulation. Thus, the underlying mechanism remains unclear. From the past few decades globin proteins have taken a vernerable positions in the cellular and
molecular biology of the three kingdoms respectively. Recently, a novel class of the globin coupled heme-containing sensor proteins has been identified in bacteria. These proteins are different from well-known heme proteins, such as hemoglobin/myoglobin (O2 carriers), cytochrome P450/peroxidases (oxygen activators), and cytochrome
c/cytochrome b5 (mediators of electron transfer). Globin-based O2 sensor proteins usually consist of an N-terminal heme-containing O2 sensor domain and a C-terminal effector
domain. The structures of globin-based sensor proteins are changed upon binding of O2 with the heme domain. This structural change influences the effector domain for proper
functioning like activation or inhibition. Against these backgrounds, the magnificent search of the Leishmania (L. major, L. infantum and L. braziliensis) genome sequence
has revealed more than 41 heme-containing protein-coding genes. Recently our laboratory has discovered a globin coupled heme containing adenylate cyclase from L.
major called HemAC-Lm, which is likely to function in cellular adaptability under various O2 tensions
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| Date |
2015-09-23
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| Type |
Thesis
NonPeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2669/1/THESIS_of_JAYASREE_ROY.pdf
Roy, Jayasree (2015) Role of active site residues in catalytic activities of heme based adenylate cyclase from Leishmania major. PhD thesis, J U. |
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| Relation |
http://www.eprints.iicb.res.in/2669/
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